| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPB49 |
| Uniprot No | Q08495 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-405aa |
| 氨基酸序列 | MERLQKQPLT SPGSVSPSRD SSVPGSPSSI VAKMDNQVLG YKDLAAIPKD KAILDIERPD LMIYEPHFTY SLLEHVELPR SRERSLSPKS TSPPPSPEVW ADSRSPGIIS QASAPRTTGT PRTSLPHFHH PETSRPDSNI YKKPPIYKQR ESVGGSPQTK HLIEDLIIES SKFPAAQPPD PNQPAKIETD YWPCPPSLAV VETEWRKRKA SRRGAEEEEE EEDDDSGEEM KALRERQREE LSKVTSNLGK MILKEEMEKS LPIRRKTRSL PDRTPFHTSL HQGTSKSSSL PAYGRTTLSR LQSTEFSPSG SETGSPGLQN GEGQRGRMDR GNSLPCVLEQ KIYPYEMLVV TNKGRTKLPP GVDRMRLERH LSAEDFSRVF AMSPEEFGKL ALWKRNELKK KASLF |
| 预测分子量 | 45,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EPB49(Dematin)重组蛋白的参考文献示例(**注：文献信息为示例性概括，建议通过学术数据库核对完整信息**)：
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1. **标题**：*"Functional characterization of recombinant human erythrocyte dematin (EPB49) and its role in membrane skeleton organization"*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌表达重组人源EPB49蛋白，分析其与红细胞膜骨架蛋白(如spectrin和actin)的相互作用，证实EPB49通过C端结构域稳定细胞膜结构，并调控细胞变形性。
2. **标题**：*"Structural insights into the actin-binding domain of dematin using X-ray crystallography"*
**作者**：Lee C, et al.
**摘要**：解析重组EPB49蛋白的actin结合结构域晶体结构，揭示其与肌动蛋白丝结合的分子机制，为理解红细胞形态异常相关疾病的分子基础提供依据。
3. **标题**：*"Role of EPB49 in diabetes-induced erythrocyte dysfunction: Recombinant protein rescue experiments"*
**作者**：Wang Y, et al.
**摘要**：探讨高糖环境下EPB49的糖化修饰对红细胞膜稳定性的影响，利用重组EPB49蛋白恢复糖尿病模型红细胞的机械性能，提示其潜在治疗价值。
4. **标题**：*"Expression and purification of recombinant dematin for functional proteomics studies"*
**作者**：Garcia R, et al.
**摘要**：优化EPB49重组蛋白在昆虫细胞中的表达与纯化流程，并利用质谱和免疫共沉淀技术筛选其相互作用蛋白网络，拓展对其在细胞信号通路中作用的认识。
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**建议**：以上文献名为概括性示例，实际研究中可通过 **PubMed/Google Scholar** 搜索关键词 *Dematin recombinant protein*、*EPB49 gene expression* 或结合具体研究领域(如红细胞疾病、细胞骨架)获取准确文献。
EPB49 (Erythrocyte Protein Band 4.9), also known as Dematin, is a cytoskeletal protein predominantly expressed in erythrocytes (red blood cells) and other tissues. It plays a critical role in maintaining membrane stability and cellular morphology by interacting with actin filaments. The protein derives its name from its migration position (Band 4.9) on SDS-PAGE gels of erythrocyte membrane proteins. Structurally, EPB49 contains a conserved actin-binding domain at its N-terminus and a C-terminal "headpiece" domain that facilitates interactions with other membrane components, such as spectrin and ankyrin.
Originally identified in the 1980s, EPB49 gained attention for its involvement in regulating the mechanical properties of red blood cells. Its ability to bundle and stabilize actin filaments contributes to the deformability and resilience of erythrocytes, which is essential for their function in oxygen transport. Studies have shown that phosphorylation of EPB49 modulates its actin-binding activity, linking it to signaling pathways that respond to cellular stress or metabolic changes.
The recombinant form of EPB49 (rEPB49) is engineered using expression systems like *E. coli* or mammalian cells to study its biochemical properties, structure-function relationships, and interactions with binding partners. Recombinant technology enables large-scale production of the protein for applications in structural biology (e.g., crystallography), drug discovery, and diagnostic assay development. Researchers also utilize rEPB49 to investigate pathological conditions, including hereditary hemolytic anemias and neurodegenerative disorders, where cytoskeletal dysregulation is implicated. Recent work explores its potential role in cancer metastasis due to its influence on cell motility and adhesion. As a tool in basic and applied research, EPB49 recombinant protein continues to provide insights into membrane biology and disease mechanisms.
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