| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KPNa5 |
| Uniprot No | O15131 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-536aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHGNLYFQGGEFDAMASPGKDNYRMKSYKNKAL NPQEMRRRREEEGIQLRKQKREEQLFKRRNVYLPRNDESMLESPIQDPDI SSTVPIPEEEVVTTDMVQMIFSNNADQQLTATQKFRKLLSKEPNPPIDQV IQKPGVVQRFVKFLERNENCTLQFEAAWALTNIASGTFLHTKVVIETGAV PIFIKLLNSEHEDVQEQAVWALGNIAGDNAECRDFVLNCEILPPLLELLT NSNRLTTTRNAVWALSNLCRGKNPPPNFSKVSPCLNVLSRLLFSSDPDVL ADVCWALSYLSDGPNDKIQAVIDSGVCRRLVELLMHNDYKVVSPALRAVG NIVTGDDIQTQVILNCSALPCLLHLLSSPKESIRKEACWTVSNITAGNRA QIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIRYLVA LGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQESKQNGIGINPYCAL IEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGVEEDDPSIVPQVDENQ QQFIFQQQEAPMDGFQL |
| 预测分子量 | 64 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KPNA5重组蛋白的模拟参考文献示例(注：文献为模拟生成，建议通过学术数据库检索真实文献)：
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1. **文献名称**：*KPNA5 facilitates nuclear import of influenza virus ribonucleoprotein complexes*
**作者**：Zhang Y, Chen L, et al.
**摘要**：本研究探讨了KPNA5在流感病毒感染中的作用，发现重组KPNA5蛋白通过与病毒核蛋白(NP)直接相互作用，介导病毒RNA复合体的核输入，促进病毒复制。实验表明，敲低KPNA5可显著抑制病毒在宿主细胞内的增殖。
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2. **文献名称**：*Expression and functional characterization of recombinant KPNA5 in leukemic cell lines*
**作者**：Smith J, Patel R, et al.
**摘要**：通过体外重组表达KPNA5蛋白，研究其在急性髓系白血病(AML)中的功能。结果显示，KPNA5过表达增强致癌转录因子(如c-Myc)的核定位，加速白血病细胞周期进程，提示其作为潜在治疗靶点。
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3. **文献名称**：*Structural insights into KPNA5-mediated nuclear transport using purified recombinant protein*
**作者**：Lee S, Kim T, et al.
**摘要**：利用重组KPNA5蛋白进行结构生物学分析，解析其与核定位信号(NLS)及核孔蛋白的结合机制。研究揭示了KPNA5特有的底物结合域，为设计靶向核转运的小分子抑制剂提供依据。
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4. **文献名称**：*KPNA5 regulates oxidative stress response by modulating Nrf2 nuclear translocation*
**作者**：Johnson A, Wang H, et al.
**摘要**：本研究发现，在氧化应激条件下，重组KPNA5蛋白表达上调，并通过介导抗氧化转录因子Nrf2的核转运，增强细胞防御能力。基因沉默KPNA5导致细胞对氧化损伤敏感性增加。
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建议通过PubMed、Google Scholar等平台以“KPNA5 recombinant protein”、“Karyopherin Alpha 5 function”为关键词检索最新文献。
**Background of KPNa5 Recombinant Protein**
KPNa5 (Karyopherin subunit alpha 5), also known as Importin-α5 or Importin alpha 6. is a member of the importin alpha family of nuclear transport proteins. These proteins play a critical role in nucleocytoplasmic transport, facilitating the movement of macromolecules between the cytoplasm and nucleus through the nuclear pore complex. KPNa5 specifically recognizes cargo proteins containing classical nuclear localization signals (NLS), binding to them and recruiting Karyopherin-β (Importin-β) to form a transport complex. This interaction enables the translocation of the cargo into the nucleus, a process essential for regulating cellular activities such as gene expression, cell cycle progression, and stress responses.
Structurally, KPNa5 contains conserved Armadillo (ARM) repeat domains that mediate NLS recognition. Its specificity for certain cargo proteins distinguishes it from other Importin-α isoforms, contributing to the diversity of nuclear transport pathways. Studies suggest KPNa5 may preferentially transport substrates involved in immune regulation, antiviral responses, and cancer-related pathways. For example, it has been implicated in the nuclear import of proteins like survivin and viral antigens, linking it to processes such as apoptosis inhibition and pathogen-host interactions.
The recombinant KPNa5 protein is typically produced in *E. coli* or mammalian expression systems, ensuring high purity and functionality for experimental applications. It serves as a vital tool in vitro to study nuclear transport mechanisms, protein-protein interactions, and NLS-dependent cargo recognition. Additionally, KPNa5 is investigated in disease contexts, including cancer and viral infections, where dysregulated nuclear transport contributes to pathogenesis. Inhibitors targeting KPNa5-cargo interactions are explored for therapeutic potential, highlighting its biomedical relevance. Overall, KPNa5 recombinant protein bridges fundamental research and translational studies, offering insights into cellular logistics and disease mechanisms.
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