| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | gABRa5 |
| Uniprot No | P31644 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-462aa |
| 氨基酸序列 | MDNGMFSGFIMIKNLLLFCISMNLSSHFGFSQMPTSSVKDETNDNITIFTRILDGLLDGYDNRLRPGLGERITQVRTDIYVTSFGPVSDTEMEYTIDVFFRQSWKDERLRFKGPMQRLPLNNLLASKIWTPDTFFHNGKKSIAHNMTTPNKLLRLEDDGTLLYTMRLTISAECPMQLEDFPMDAHACPLKFGSYAYPNSEVVYVWTNGSTKSVVVAEDGSRLNQYHLMGQTVGTENISTSTGEYTIMTAHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGWAWDGKKALEAAKIKKKREVILNKSTNAFTTGKMSHPPNIPKEQTPAGTSNTTSVSVKPSEEKTSESKKTYNSISKIDKMSRIVFPVLFGTFNLVYWATYLNREPVIKGAASPK |
| 预测分子量 | 52,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GABRA5重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*"Expression and Purification of Recombinant GABAA Receptor α5 Subunit in Insect Cells"*
**作者**：Smith J, et al.
**摘要**：研究报道了利用杆状病毒-昆虫细胞系统成功表达并纯化GABRA5重组蛋白，通过亲和层析和尺寸排阻色谱获得高纯度蛋白，为后续结构解析及药物筛选奠定基础。
2. **文献名称**：*"Structural Insights into GABAA Receptor α5 Subunit by Cryo-EM: Implications for Benzodiazepine Binding"*
**作者**：Chen L, et al.
**摘要**：利用冷冻电镜技术解析了GABRA5重组蛋白的原子级结构，揭示了其与苯二氮䓬类药物的结合位点特征，为开发针对焦虑和认知障碍的亚型选择性药物提供依据。
3. **文献名称**：*"Functional Characterization of Recombinant GABRA5 in Lipid Bilayer Systems"*
**作者**：Wang Y, et al.
**摘要**：通过将重组GABRA5蛋白与β3/γ2亚基共表达于人工脂质膜，验证了其形成功能性氯离子通道的能力，并发现其电生理特性与海马神经元中的天然受体高度一致。
*注：上述文献为示例，实际研究中建议通过PubMed或Web of Science检索最新论文。若需具体文献，可提供更详细的研究方向(如结构、药物开发等)。*
gABRa5. or gamma-aminobutyric acid type A receptor subunit alpha-5. is a critical component of GABA_A receptors, the primary inhibitory neurotransmitter receptors in the central nervous system. These receptors are ligand-gated chloride channels that mediate fast synaptic inhibition, regulating neuronal excitability. The α5 subunit, encoded by the *GABRA5* gene, is predominantly expressed in the hippocampus, cortex, and olfactory bulb, where it contributes to tonic inhibition and modulates cognitive processes such as learning and memory.
Recombinant gABRa5 proteins are engineered to study the structure, function, and pharmacology of α5-containing GABA_A receptors. These receptors are of particular interest due to their role in neurodevelopmental and psychiatric disorders. For example, reduced α5 subunit expression or function has been linked to cognitive deficits in Down syndrome, while its overexpression is associated with anxiety-like behaviors and epilepsy. Additionally, α5-selective negative allosteric modulators are being explored for cognitive enhancement in Alzheimer’s disease.
The recombinant gABRa5 protein is typically produced using heterologous expression systems (e.g., HEK293 or insect cells) to ensure proper post-translational modifications and ligand-binding activity. It often includes tags (e.g., His-tag) for purification and detection. Researchers use this protein to investigate receptor assembly, screen for subtype-specific drugs, or analyze interactions with endogenous modulators like neurosteroids. Its study also aids in understanding how α5-containing receptors influence synaptic plasticity and network oscillations, providing insights into therapeutic targeting while minimizing side effects associated with broader GABA_A receptor modulation.
×
