| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CKAP5 |
| Uniprot No | Q14008 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-2032aa |
| 氨基酸序列 | MGDDSEWLKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGIEICLMYIEIEKGEAVQEELLKGLDNKNPKIIVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINSVQLKELEEEWVKLPTSAPRPTRFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALESVEVLIKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELIHGKKAGLAADKKEFKPLPGRTAASGAAGDKDTKDISAPKPGPLKKAPAAKAGGPPKKGKPAAPGGAGNTGTKNKKGLETKEIVEPELSIEVCEEKASAVLPPTCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQVVLDGLVDKIGDVKCGNNAKEAMTAIAEACMLPWTAEQVVSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTAAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQSPPAPTRGISKHSTSGTDEGEDGDEPDDGSNDVVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAGIINDAKFIQPNIGELPTALKGRLNDSNKILVQQTLNILQQLAVAMGPNIKQHVKNLGIPIITVLGDSKNNVRAAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPHLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKVNMPAKPAPPTKATSKPMGGSAPAKFQPASAPAEDCISSSTEPKPDPKKAKAPGLSSKAKSAQGKKMPSKTSLKEDEDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWLTLRFFDTNTSVLMKALEYLKLLFTLLSEEEYHLTENEASSFIPYLVVKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAVHIGDRDNAVRNAALNTIVTVYNVHGDQVFKLIGNLSEKDMSMLEERIKRSAKRPSAAPIKQVEEKPQRAQNISSNANMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDNGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEIIKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVIRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRISTSTGISPQMEVTCVPTPTSTVSSIGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTHSSGTVTSSSSTANIDDLKKRLERIKSSRK |
| 预测分子量 | 225,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与CKAP5(XMAP215/ch-TOG家族)重组蛋白相关的文献摘要概括：
1. **文献名称**：*Structural analysis of the microtubule-associated protein CKAP5 (XMAP215) in microtubule nucleation*
**作者**：Al-Bassam J et al.
**摘要**：通过重组CKAP5蛋白的体外实验，揭示了其C端结构域通过微管成核机制促进微管聚合的分子机制，证明其作为微管正端追踪蛋白的功能。
2. **文献名称**：*Reconstitution of microtubule dynamics regulation by CKAP5 and CLASP proteins*
**作者**：Brouhard GJ et al.
**摘要**：利用重组CKAP5蛋白与CLASP蛋白共表达系统，阐明二者协同调控微管动态稳定性，维持有丝分裂纺锤体结构的分子机制。
3. **文献名称**：*CKAP5 promotes colorectal cancer progression through stabilizing β-catenin*
**作者**：Ohmachi T et al.
**摘要**：研究发现重组CKAP5蛋白在结直肠癌细胞中异常高表达，通过稳定β-catenin信号通路促进肿瘤侵袭转移，提示其作为潜在治疗靶点。
4. **文献名称**：*Domain mapping of CKAP5 reveals distinct roles in microtubule growth and organization*
**作者**：van der Vaart B et al.
**摘要**：通过重组CKAP5截短体功能实验，鉴定其N端TOG结构域对微管延伸的关键作用，而C端结构域参与微管束形成。
(注：以上为模拟简化摘要，实际文献需通过PubMed等数据库检索确认。)
CKAP5 (Cytoskeleton-Associated Protein 5), also known as ch-TOG or colonic and hepatic tumor overexpressed gene, is a conserved microtubule-associated protein critical for regulating cytoskeletal dynamics. It plays a vital role in mitosis by stabilizing spindle microtubules and promoting their assembly, ensuring accurate chromosome segregation during cell division. CKAP5 interacts with the γ-tubulin ring complex (γ-TuRC) to facilitate microtubule nucleation and is essential for maintaining mitotic spindle integrity. Dysregulation of CKAP5 has been linked to genomic instability, aneuploidy, and cancer progression, as its overexpression is observed in various tumors, including colorectal and hepatic cancers.
Recombinant CKAP5 protein, produced via heterologous expression systems like *E. coli* or mammalian cells, retains functional domains necessary for microtubule binding and regulatory activity. This engineered protein is widely used in biochemical assays to study microtubule polymerization kinetics, spindle assembly mechanisms, and interactions with other mitotic regulators. Its applications extend to drug discovery, where it serves as a target for screening anticancer compounds aimed at disrupting microtubule dynamics in proliferating cells. Researchers also employ CKAP5 recombinant protein to investigate its role in cellular stress responses, such as resistance to paclitaxel or other microtubule-targeting chemotherapeutics. Structural studies using recombinant CKAP5 have elucidated its domain architecture, including TOG (Tumor Overexpressed Gene) domains that mediate tubulin binding, providing insights into evolutionary conservation across species. As a tool, it bridges molecular biology and clinical research, offering potential pathways for therapeutic intervention in cancers driven by cytoskeletal dysregulation.
×
