| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NRD1 |
| Uniprot No | O43847 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1151aa |
| 氨基酸序列 | MLRRVTVAAVCATRRKLCEAGRELAALWGIETRGRCEDSAAARPFPILAMPGRNKAKSTCSCPDLQPNGQDLGENSRVARLGADESEEEGRRGSLSNAGDPEIVKSPSDPKQYRYIKLQNGLQALLISDLSNMEGKTGNTTDDEEEEEVEEEEEDDDEDSGAEIEDDDEEGFDDEDEFDDEHDDDLDTEDNELEELEERAEARKKTTEKQSAAALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLKHEPRKNNIDTHARLREFWMRYYSSHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPRPNFGHLTDPFDTPAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSFLRKKCWALALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAYTVFQYLKMLQKLGPEKRIFEEIRKIEDNEFHYQEQTDPVEYVENMCENMQLYPLQDILTGDQLLFEYKPEVIGEALNQLVPQKANLVLLSGANEGKCDLKEKWFGTQYSIEDIENSWAELWNSNFELNPDLHLPAENKYIATDFTLKAFDCPETEYPVKIVNTPQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNHKLPLLFQLIIDYLAEFNSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYARWSMIDKYQALMDGLSLESLLSFVKEFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFKPLEQEMPVQFQVVELPSGHHLCKVKALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILGFSVTVGTQATKYNSEVVDKKIEEFLSSFEEKIENLTEEAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVNWFKAHRGPGSKMLSVHVVGYGKYELEEDGTPSSEDSNSSCEVMQLTYLPTSPLLADCIIPITDIRAFTTTLNLLPYHKIVK |
| 预测分子量 | 131,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NRD1重组蛋白的3篇代表性文献示例(文献信息为模拟虚构，供参考)：
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1. **文献名称**：*Structural Insights into the Catalytic Mechanism of Recombinant NRD1 Metalloprotease*
**作者**：Li, X. et al.
**摘要**：本研究报道了重组NRD1蛋白的晶体结构解析，揭示了其锌离子结合域和底物识别机制，为理解其参与前体蛋白水解加工的催化活性提供了分子基础。
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2. **文献名称**：*Expression and Functional Characterization of NRD1 in Neuronal Development*
**作者**：Martinez, R. et al.
**摘要**：通过大肠杆菌系统表达并纯化重组NRD1蛋白，发现其通过调控Notch信号通路影响神经元分化，提示其在神经发育疾病中的潜在作用。
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3. **文献名称**：*NRD1-Mediated Extracellular Matrix Remodeling in Cancer Metastasis*
**作者**：Chen, H. & Wang, Y.
**摘要**：利用哺乳动物细胞表达的重组NRD1蛋白，证明其通过降解特定基质蛋白促进肿瘤细胞迁移，为靶向NRD1的抗癌策略提供了实验依据。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“NRD1 recombinant protein”“Nardilysin expression”等进一步筛选。
**Background of NRD1 Recombinant Protein**
NRD1 (Nardilysin), also known as NRD convertase, is a zinc-dependent metalloendopeptidase belonging to the M16 family. It plays a regulatory role in proteolytic processing by selectively cleaving substrates at specific N-terminal arginine or lysine residues. Structurally, NRD1 contains a unique N-terminal domain involved in substrate recognition and a C-terminal catalytic domain with a conserved zinc-binding motif (HEXXH). The enzyme is implicated in diverse biological processes, including neuropeptide maturation, growth factor signaling, and cellular differentiation.
Recombinant NRD1 protein is engineered for research applications to study its enzymatic activity and interaction networks. It is typically produced using *E. coli* or mammalian expression systems, ensuring high purity and bioactivity through affinity chromatography. The recombinant form retains catalytic efficiency and substrate specificity, enabling *in vitro* studies on its role in processing substrates like glucagon, neurotensin, and amyloid precursor protein (APP).
Research highlights NRD1's involvement in neurodegenerative diseases (e.g., Alzheimer’s via APP processing), cancer (modulating growth factor pathways), and metabolic disorders (insulin regulation). Its dual role as a chaperone and protease further underscores its therapeutic potential. Recombinant NRD1 serves as a critical tool for elucidating molecular mechanisms, screening inhibitors, and developing targeted therapies.
In summary, NRD1 recombinant protein bridges structural, functional, and translational studies, offering insights into its pathophysiological roles and therapeutic applications.
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