| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NT5C1B |
| Uniprot No | Q96P26 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-610aa |
| 氨基酸序列 | MSQTSLKQKKNEPGMRSSKESLEAEKRKESDKTGVRLSNQMRRAVNPNHSLRCCPFQGHSSCRRCLCAAEGTALGPCHTIRIYIHMCLLWEQGQQITMMRGSQESSLRKTDSRGYLVRSQWSRISRSPSTKAPSIDEPRSRNTSAKLPSSSTSSRTPSTSPSLHDSSPPPLSGQPSLQPPASPQLPRSLDSRPPTPPEPDPGSRRSTKMQENPEAWAQGIVREIRQTRDSQPLEYSRTSPTEWKSSSQRRGIYPASTQLDRNSLSEQQQQQREDEDDYEAAYWASMRSFYEKNPSCSRPWPPKPKNAITIALSSCALFNMVDGRKIYEQEGLEKYMEYQLTNENVILTPGPAFRFVKALQYVNARLRDLYPDEQDLFDIVLMTNNHAQVGVRLINSVNHYGLLIDRFCLTGGKDPIGYLKAYLTNLYIAADSEKVQEAIQEGIASATMFDGAKDMAYCDTQLRVAFDGDAVLFSDESEHFTKEHGLDKFFQYDTLCESKPLAQGPLKGFLEDLGRLQKKFYAKNERLLCPIRTYLVTARSAASSGARVLKTLRRWGLEIDEALFLAGAPKSPILVKIRPHIFFDDHMFHIEGAQRLGSIAAYGFNKKFSS |
| 预测分子量 | 68,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NT5C1B重组蛋白的3篇代表性文献示例(注：内容为模拟概括，具体文献需根据实际检索结果调整)：
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1. **文献名称**：*NT5C1B promotes 5-fluorouracil resistance in colorectal cancer through regulating nucleotide metabolism*
**作者**：Zhang Y, et al.
**摘要**：本研究通过在大肠杆菌中表达重组NT5C1B蛋白，揭示了其在结直肠癌细胞中对5-氟尿嘧啶(5-FU)耐药性的调控作用。实验表明，NT5C1B通过水解活性代谢药物中间体，降低细胞内药物毒性，从而增强癌细胞存活率。
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2. **文献名称**：*Structural and functional characterization of recombinant human NT5C1B*
**作者**：Li H, et al.
**摘要**：作者利用昆虫细胞系统表达并纯化NT5C1B重组蛋白，通过X射线晶体学解析其三维结构，阐明了其底物结合域的关键氨基酸残基，为靶向抑制剂设计提供了结构基础。
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3. **文献名称**：*NT5C1B overexpression correlates with hepatocellular carcinoma progression and poor prognosis*
**作者**：Wang X, et al.
**摘要**：研究通过构建NT5C1B重组蛋白并制备特异性抗体，发现其在肝细胞癌中异常高表达，体外实验证实其通过调控核苷酸池平衡促进癌细胞增殖和转移。
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4. **文献名称**：*Optimization of recombinant NT5C1B expression in E. coli for enzymatic assays*
**作者**：Smith J, et al.
**摘要**：本文系统优化了NT5C1B重组蛋白在大肠杆菌中的表达条件，通过亲和层析和凝胶电泳验证高纯度蛋白的获取，并建立其体外酶活检测体系，为高通量药物筛选奠定基础。
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(注：以上文献信息为示例，实际引用需以真实发表内容为准。)
**Background of NT5C1B Recombinant Protein**
NT5C1B (5'-Nucleotidase, Cytosolic IB), a member of the 5'-nucleotidase family, is an enzyme that catalyzes the hydrolysis of nucleoside monophosphates (e.g., AMP, IMP) into nucleosides and inorganic phosphate. This activity regulates intracellular nucleotide pools, balancing purine and pyrimidine metabolism, and influences nucleotide-dependent signaling pathways. NT5C1B is predominantly expressed in the cytoplasm and has been implicated in cellular processes such as energy homeostasis, DNA repair, and cell proliferation.
Recombinant NT5C1B protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian systems, followed by purification to ensure high specificity and activity. Its recombinant form enables detailed biochemical and structural studies, including substrate specificity analyses, inhibitor screening, and mechanistic investigations into nucleotide metabolism dysregulation.
Research highlights NT5C1B's role in disease contexts. In cancer, NT5C1B overexpression or mutations (e.g., in acute lymphoblastic leukemia) correlate with drug resistance, possibly by altering nucleotide salvage pathways or detoxifying nucleoside analog chemotherapeutics. Additionally, its interaction with metabolic disorders and inflammatory conditions, such as atherosclerosis, underscores its broader pathophysiological relevance.
As a therapeutic target, NT5C1B inhibitors are being explored to counteract chemoresistance in malignancies. Structurally, the protein features conserved nucleotidase domains and metal-binding sites critical for catalysis, with recombinant variants often including tags (e.g., His-tag) for purification. Studies leveraging recombinant NT5C1B continue to advance understanding of nucleotide metabolism in health and disease, offering potential diagnostic or therapeutic applications.
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