| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXE1 |
| Uniprot No | O00358 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373aa |
| 氨基酸序列 | MTAESGPPPP QPEVLATVKE ERGETAAGAG VPGEATGRGA GGRRRKRPLQ RGKPPYSYIA LIAMAIAHAP ERRLTLGGIY KFITERFPFY RDNPKKWQNS IRHNLTLNDC FLKIPREAGR PGKGNYWALD PNAEDMFESG SFLRRRKRFK RSDLSTYPAY MHDAAAAAAA AAAAAAAAAI FPGAVPAARP PYPGAVYAGY APPSLAAPPP VYYPAASPGP CRVFGLVPER PLSPELGPAP SGPGGSCAFA SAGAPATTTG YQPAGCTGAR PANPSAYAAA YAGPDGAYPQ GAGSAIFAAA GRLAGPASPP AGGSSGGVET TVDFYGRTSP GQFGALGACY NPGGQLGGAS AGAYHARHAA AYPGGIDRFV SAM |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXE1重组蛋白的3篇代表性文献的简要整理(注：部分内容基于真实研究概括，文献标题和作者为示例性虚构，仅供参考)：
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1. **文献名称**: *Functional characterization of recombinant FOXE1 in thyroid-specific gene regulation*
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌表达系统制备了重组FOXE1蛋白，并利用凝胶迁移实验(EMSA)证明其与甲状腺球蛋白(TG)基因启动子区的特异性结合能力。进一步发现磷酸化修饰可增强FOXE1的转录激活功能。
2. **文献名称**: *Structural insights into FOXE1 DNA-binding domain by X-ray crystallography*
**作者**: Tanaka K, et al.
**摘要**: 作者通过重组表达并纯化人源FOXE1的DNA结合结构域(氨基酸1-100)，利用X射线晶体学解析其三维结构(分辨率2.1Å)，揭示了叉头结构域中关键残基与DNA相互作用的分子机制。
3. **文献名称**: *FOXE1 mutations disrupt cellular localization of recombinant protein in vitro*
**作者**: Rodriguez S, et al.
**摘要**: 研究构建了携带先天性甲状腺缺如相关突变(如R73S)的FOXE1重组蛋白，发现突变体在HeLa细胞中的核定位效率显著降低，提示其病理机制与蛋白亚细胞分布异常相关。
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**注**：以上文献为示例，实际引用时请通过PubMed或Web of Science以关键词“FOXE1 recombinant”检索最新研究，并核对原文内容。
FOXE1 (Forkhead Box E1), also known as Thyroid Transcription Factor 2 (TTF-2), is a member of the forkhead box (FOX) family of transcription factors, characterized by a conserved DNA-binding domain termed the "forkhead domain." This protein plays critical roles in embryonic development, particularly in thyroid morphogenesis, palate formation, and hair follicle differentiation. FOXE1 regulates gene expression by binding to specific DNA sequences, influencing cellular proliferation, migration, and differentiation pathways.
Recombinant FOXE1 protein is engineered using molecular cloning techniques, where the FOXE1 gene is inserted into expression vectors (e.g., bacterial, mammalian, or insect cell systems) to produce purified, functional protein. This lab-generated protein retains the biological activity of native FOXE1. including DNA-binding and transcriptional regulatory functions. Researchers utilize recombinant FOXE1 to study its interactions with target genes, signaling pathways, and epigenetic modifiers in developmental and disease contexts.
Clinically, FOXE1 mutations are linked to congenital hypothyroidism, Bamforth-Lazarus syndrome, and susceptibility to thyroid cancer. Recombinant FOXE1 aids in elucidating molecular mechanisms underlying these conditions, serving as a tool for in vitro assays, protein-DNA interaction studies, and therapeutic exploration. Its applications extend to drug screening for thyroid disorders and regenerative medicine research. Despite progress, challenges remain in understanding tissue-specific FOXE1 regulation and translating findings into clinical interventions, making recombinant FOXE1 a vital resource for ongoing biomedical investigations.
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