| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXE3 |
| Uniprot No | Q13461 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-319aa |
| 氨基酸序列 | MAGRSDMDPP AAFSGFPALP AVAPSGPPPS PLAGAEPGRE PEEAAAGRGE AAPTPAPGPG RRRRRPLQRG KPPYSYIALI AMALAHAPGR RLTLAAIYRF ITERFAFYRD SPRKWQNSIR HNLTLNDCFV KVPREPGNPG KGNYWTLDPA AADMFDNGSF LRRRKRFKRA ELPAHAAAAP GPPLPFPYAP YAPAPGPALL VPPPSAGPGP SPPARLFSVD SLVNLQPELA GLGAPEPPCC AAPDAAAAAF PPCAAAASPP LYSQVPDRLV LPATRPGPGP LPAEPLLALA GPAAALGPLS PGEAYLRQPG FASGLERYL |
| 预测分子量 | 33,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXE3重组蛋白的示例参考文献(注：以下内容为虚构示例，仅供格式参考，实际文献需通过学术数据库查询)：
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1. **"Functional characterization of recombinant FOXE3 in lens development"**
*Author: Smith A, et al. (2017)*
*摘要*：本研究通过在大肠杆菌中表达重组FOXE3蛋白，验证了其DNA结合能力及对α-晶状体蛋白基因启动子的调控作用，揭示了FOXE3在晶状体分化中的关键功能。
2. **"Structural analysis of FOXE3 forkhead domain by X-ray crystallography"**
*Author: Zhang L, et al. (2020)*
*摘要*：利用重组FOXE3蛋白的晶体结构解析，发现其叉头结构域中特定氨基酸突变与先天性白内障相关，为疾病机制提供了结构生物学依据。
3. **"FOXE3 recombinant protein rescues corneal defects in zebrafish models"**
*Author: Tanaka K, et al. (2019)*
*摘要*：在斑马鱼模型中，外源性重组FOXE3蛋白恢复了角膜上皮细胞分化异常，证明其在角膜发育中的治疗潜力。
4. **"In vitro interaction screening of FOXE3 with epigenetic modifiers"**
*Author: Patel R, et al. (2021)*
*摘要*：通过重组FOXE3与组蛋白修饰酶的体外结合实验，发现其与HDAC3的相互作用可能调控晶状体发育的表观遗传网络。
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建议通过PubMed或Google Scholar搜索关键词“FOXE3 recombinant protein”或“FOXE3 purification/function”获取真实文献。
**Background of FOXE3 Recombinant Protein**
FOXE3 (Forkhead Box E3) is a transcription factor belonging to the forkhead/winged-helix family, characterized by a conserved DNA-binding domain. It plays a critical role in ocular development, particularly in lens formation and maintenance of lens transparency. Mutations in the *FOXE3* gene are associated with congenital eye disorders, including primary aphakia, microphthalmia, and cataracts, highlighting its essential function in eye morphogenesis.
The FOXE3 protein regulates gene expression by binding to specific DNA sequences, influencing cellular differentiation and proliferation during embryogenesis. Its activity is tightly linked to the development of the lens placode and subsequent maturation of lens epithelial cells. Studies in animal models, such as mice and zebrafish, have demonstrated that FOXE3 loss-of-function leads to severe ocular malformations, underscoring its evolutionary conservation.
Recombinant FOXE3 protein is engineered using biotechnological platforms, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. This recombinant form enables *in vitro* and *in vivo* studies to dissect FOXE3's molecular mechanisms, including its interactions with co-factors or DNA targets. Researchers utilize it to explore pathways involved in lens development, disease modeling, and potential therapeutic strategies for eye disorders.
Additionally, FOXE3 recombinant protein serves as a tool for antibody production, structural studies, and high-throughput screening assays. Its applications extend to investigating epigenetic regulation and signaling cascades disrupted in congenital eye diseases. By providing a purified, functional form of FOXE3. this recombinant protein accelerates research into developmental biology and ophthalmology, bridging gaps between genetic findings and mechanistic insights.
In summary, FOXE3 recombinant protein is a vital resource for understanding ocular development and pathology, offering avenues for translational research aimed at addressing vision-impairing conditions.
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