| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXA3 |
| Uniprot No | P55318 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-350aa |
| 氨基酸序列 | 29 amino acid tag-GEFLGSVKMEAHDLAEWSYYPEAGEVYSPVTPVPTMAPLNSYMTLN PLSSPYPPGGLPASPLPSGPLAPPAPAAPLGPTFPGLGVSGGSSSSGYGA PGPGLVHGKEMPKGYRRPLAHAKPPYSYISLITMAIQQAPGKMLTLSEIY QWIMDLFPYYRENQQRWQNSIRHSLSFNDCFVKVARSPDKPGKGSYWALH PSSGNMFENGCYLRRQKRFKLEEKVKKGGSGAATTTRNGTGSAASTTTPA ATVTSPPQPPPPAPEPEAQGGEDVGALDCGSPASSTPYFTGLELPGELKL DAPYNFNHPFSINNLMSEQTPAPPKLDVGFGGYGAEGGEPGVYYQGLYSR SLLNASLEESGGGGSPGRRRRRRRRRRR |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXA3重组蛋白的模拟参考文献示例(注：内容为示例性概括，非真实文献)：
1. **"Recombinant FOXA3 Protein Purification and DNA Binding Analysis"**
*作者：Smith J, et al.*
摘要：研究报道了通过大肠杆菌表达系统制备重组FOXA3蛋白的优化方法，并利用凝胶迁移实验(EMSA)验证其与靶基因启动子区域的DNA结合能力，揭示了FOXA3在代谢调控中的作用机制。
2. **"Functional Characterization of FOXA3 in Hepatic Gene Regulation"**
*作者：Lee H, et al.*
摘要：通过体外表达纯化的重组FOXA3蛋白，结合染色质免疫沉淀(ChIP)技术，证明FOXA3直接调控肝脏特异性基因(如ApoB)的表达，并参与脂质代谢通路。
3. **"FOXA3 Recombinant Protein Enhances Cellular Reprogramming Efficiency"**
*作者：Zhang R, et al.*
摘要：研究利用重组FOXA3蛋白与转录因子组合，显著提高了体细胞向肝细胞转分化的效率，表明其在再生医学中的潜在应用价值。
4. **"Structural Insights into FOXA3-DNA Interaction via Recombinant Protein Crystallography"**
*作者：Wang Y, et al.*
摘要：通过重组FOXA3蛋白的结晶和结构解析，揭示了其叉头结构域与DNA结合的分子机制，为设计靶向FOXA3的小分子抑制剂提供了结构基础。
(注：以上内容为学术示例，实际文献需通过数据库如PubMed、Web of Science检索核实。)
FOXA3 (Forkhead Box A3), a member of the forkhead box (Fox) transcription factor family, plays critical roles in organ development, metabolism regulation, and cellular differentiation. As part of the FoxA subfamily (FoxA1. A2. A3), it binds DNA through a conserved winged-helix "forkhead" domain, facilitating chromatin remodeling and transcriptional activation of target genes. FOXA3 is predominantly expressed in metabolic tissues such as the liver, pancreas, and adipose tissue, where it regulates glucose and lipid metabolism, energy homeostasis, and hormone signaling. Its involvement in metabolic pathways has linked it to diseases like obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD).
Recombinant FOXA3 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce functional, purified protein for research applications. The recombinant form typically retains DNA-binding activity and interaction capabilities with co-regulators, enabling in vitro studies of gene regulation mechanisms. Researchers utilize it to investigate FOXA3's role in metabolic reprogramming, insulin signaling, and tissue-specific gene expression through techniques like electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and cell culture experiments.
Studies with recombinant FOXA3 have highlighted its dual role in metabolism: it promotes lipogenesis and suppresses β-oxidation in the liver under certain conditions, while also influencing pancreatic β-cell function and adipocyte differentiation. Its context-dependent activity underscores its potential as a therapeutic target for metabolic disorders. However, conflicting findings regarding its pro- or anti-metabolic disease effects emphasize the need for further mechanistic exploration. Recombinant FOXA3 thus serves as a vital tool for dissecting molecular pathways in metabolism and disease.
×
