| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACSL4 |
| Uniprot No | O60488 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-670aa |
| 氨基酸序列 | MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFG KKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSG LTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVH GLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEG FEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMH HSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIG YSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQE MNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLS GGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAP LICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDY SVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAA LKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVDICN NPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKL KRKELRNHYLKDIERMYGGK |
| 预测分子量 | 101 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于ACSL4重组蛋白的参考文献示例(内容为模拟概括，仅供参考)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of Human Acyl-CoA Synthetase 4 (ACSL4) in Escherichia coli"*
**作者**: Maloberti, P.M., et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组人源ACSL4蛋白，分析了其酶动力学特性，证实其对长链多不饱和脂肪酸(如花生四烯酸)的优先激活作用，为体外研究ACSL4的代谢功能提供了工具。
2. **文献名称**: *"ACSL4-Dependent Ferroptosis in Cancer Cells: Role of Recombinant ACSL4 in Lipid Peroxidation"*
**作者**: Kagan, V.E., et al.
**摘要**: 通过体外重组ACSL4蛋白实验，揭示了ACSL4通过促进细胞膜磷脂中多不饱和脂肪酸的酯化，增强铁死亡(ferroptosis)敏感性，为癌症治疗中靶向ACSL4的策略提供了机制依据。
3. **文献名称**: *"Structural Insights into ACSL4 Isoforms Using Recombinant Protein Crystallography"*
**作者**: Yuan, H., et al.
**摘要**: 通过重组ACSL4蛋白的晶体结构解析，发现其不同剪接变体的底物结合口袋差异，阐明了特定异构体在阿尔茨海默病相关脂质代谢异常中的潜在作用。
4. **文献名称**: *"ACSL4 as a Biomarker in Triple-Negative Breast Cancer: Validation via Recombinant Protein-Based Assays"*
**作者**: Li, Y., et al.
**摘要**: 利用重组ACSL4蛋白建立高通量酶活性检测方法，证实其在三阴性乳腺癌细胞中高表达且与患者预后不良相关，提示其作为治疗靶点的潜力。
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*注：以上文献为示例性内容，实际引用时需根据真实文献调整。建议通过PubMed、Web of Science等数据库检索关键词“ACSL4 recombinant”“ACSL4 ferroptosis”获取准确信息。*
**Background of ACSL4 Recombinant Protein**
ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4) is a key enzyme in lipid metabolism, catalyzing the activation of long-chain fatty acids into acyl-CoA esters. This reaction is critical for fatty acid degradation, membrane phospholipid remodeling, and biosynthesis of lipid signaling molecules. Structurally, ACSL4 contains a conserved AMP-binding domain and exhibits substrate specificity for polyunsaturated fatty acids (PUFAs), linking it to cellular processes like inflammation, ferroptosis, and cancer progression. Dysregulation of ACSL4 has been implicated in metabolic disorders, neurodegenerative diseases, and malignancies, particularly in hormone-related cancers (e.g., breast, prostate) and hepatocellular carcinoma.
Recombinant ACSL4 protein is produced via genetic engineering, often using *E. coli* or mammalian expression systems, to enable functional studies. Its purification typically involves affinity tags (e.g., His-tag) for high yield and purity. Researchers employ ACSL4 recombinant protein to investigate enzymatic activity, lipid-protein interactions, and mechanisms underlying diseases. For example, ACSL4’s role in ferroptosis—a regulated cell death pathway driven by lipid peroxidation—has spurred interest in targeting it for cancer therapy. Studies also explore its involvement in modulating lipid droplets and membrane dynamics in metabolic syndromes.
Furthermore, ACSL4 serves as a biomarker in certain cancers, with overexpression correlating with aggressive phenotypes and drug resistance. Its recombinant form facilitates drug screening, antibody development, and structural studies to design inhibitors. Recent advances highlight ACSL4’s dual role in health and disease, emphasizing its potential as a therapeutic target. Ongoing research aims to unravel tissue-specific functions and regulatory networks, positioning ACSL4 recombinant protein as a vital tool for biomedical discovery.
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