| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSSP |
| Uniprot No | Q9NQE7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-514aa |
| 氨基酸序列 | SLLRRL GEHIQQFQES SAQGLGLSLG PGAAALPKVG WLEQLLDPFN VSDRRSFLQR YWVNDQHWVG QDGPIFLHLG GEGSLGPGSV MRGHPAALAP AWGALVISLE HRFYGLSIPA GGLEMAQLRF LSSRLALADV VSARLALSRL FNISSSSPWI CFGGSYAGSL AAWARLKFPH LIFASVASSA PVRAVLDFSE YNDVVSRSLM STAIGGSLEC RAAVSVAFAE VERRLRSGGA AQAALRTELS ACGPLGRAEN QAELLGALQA LVGGVVQYDG QTGAPLSVRQ LCGLLLGGGG NRSHSTPYCG LRRAVQIVLH SLGQKCLSFS RAETVAQLRS TEPQLSGVGD RQWLYQTCTE FGFYVTCENP RCPFSQLPAL PSQLDLCEQV FGLSALSVAQ AVAQTNSYYG GQTPGANKVL FVNGDTDPWH VLSVTQALGS SESTLLIRTG SHCLDMAPER PSDSPSLRLG RQNIFQQLQT WLKLAKESQI KGEV |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSSP(胸腺特异性丝氨酸蛋白酶)重组蛋白研究的虚构参考文献示例(仅供参考，非真实文献)：
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1. **文献名称**：*Expression and Functional Analysis of Recombinant TSSP in Antigen Presentation*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌系统中表达并纯化重组TSSP蛋白，证实其在体外具有蛋白酶活性，并参与MHC-II类分子抗原加工过程，为T细胞发育研究提供工具。
2. **文献名称**：*Structural Insights into TSSP Recombinant Protein via X-ray Crystallography*
**作者**：Li H, et al.
**摘要**：通过哺乳动物细胞表达系统获得高纯度TSSP重组蛋白，解析其晶体结构，揭示活性位点关键氨基酸残基，为靶向药物设计奠定基础。
3. **文献名称**：*Role of Recombinant TSSP in Autoimmune Disease Models*
**作者**：Garcia R, et al.
**摘要**：利用昆虫细胞表达重组TSSP，发现其过表达可加剧小鼠自身免疫性关节炎，提示TSSP可能作为潜在治疗靶点。
4. **文献名称**：*Optimization of TSSP Recombinant Production in Yeast Systems*
**作者**：Wang Y, et al.
**摘要**：通过毕赤酵母系统优化TSSP重组蛋白表达条件，提高产量并验证其酶动力学特性，为规模化制备提供可行方案。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词“TSSP recombinant protein”或“thymus-specific serine protease expression”。
**Background of TSSP Recombinant Protein**
TSSP (testis-specific serine protease) is a protein predominantly expressed in the male reproductive system, particularly in germ cells during spermatogenesis. First identified in mice and later in humans, TSSP belongs to the serine protease family, enzymes characterized by a catalytic triad of serine, histidine, and aspartic acid. Its testis-specific expression suggests a critical role in sperm maturation, fertilization, or related reproductive processes. However, its precise physiological function remains under investigation, partly due to challenges in studying endogenous TSSP in vivo.
Recombinant TSSP protein is produced using genetic engineering techniques, often via heterologous expression systems such as *E. coli*, yeast, or mammalian cell lines. This approach enables large-scale production of the protein for functional and structural studies. The recombinant form typically retains the enzymatic activity of native TSSP, allowing researchers to explore its substrate specificity, regulatory mechanisms, and potential interactions with other proteins in reproductive pathways.
Interest in TSSP stems from its potential implications in male infertility and contraceptive development. Mutations or dysregulation of TSSP have been linked to impaired spermatogenesis in animal models, highlighting its biological significance. Additionally, its restricted tissue expression makes it a promising target for non-hormonal male contraceptives with fewer systemic side effects.
Despite progress, challenges persist, including optimizing recombinant TSSP stability, post-translational modifications (e.g., glycosylation), and resolving structural details to aid drug design. Ongoing research combines biochemical assays, crystallography, and transgenic models to unravel TSSP's role in reproduction and its therapeutic potential.
In summary, TSSP recombinant protein serves as a vital tool for decoding the molecular mechanisms of fertility and advancing reproductive health solutions.
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