| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPPA3 |
| Uniprot No | Q6W0C5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-159aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMDPSQFNPTYIPGSPQMLTEENSRDDS GASQISSETLIKNLSNLTINASSESVSPLSEALLRRESVGAAVLREIEDE WLYSRRGVRTLLSVQREKMARLRYMLLGGVRTHERRPTNKEPKGVKKESR PFKCPCSFCVSNGWDPSENARIGNQDTKPLQP |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPPA3重组蛋白的3篇参考文献示例(内容为虚构，供参考)：
1. **文献名称**: "Recombinant DPPA3 Protein Enhances Somatic Cell Reprogramming Efficiency"
**作者**: Takahashi, Y. et al.
**摘要**: 研究报道了通过大肠杆菌表达系统成功制备重组DPPA3蛋白，并验证其通过调控DNA甲基化显著提高体细胞向多能干细胞重编程的效率，为再生医学提供新工具。
2. **文献名称**: "Structural and Functional Characterization of DPPA3 in Germ Cell Development"
**作者**: Smith, R. & Lee, J.
**摘要**: 该研究解析了重组DPPA3蛋白的晶体结构，揭示其通过结合组蛋白H3K9me2修饰参与生殖细胞表观遗传重塑，为理解早期胚胎发育机制提供依据。
3. **文献名称**: "DPPA3 Recombinant Protein Promotes Epigenetic Stability in Induced Pluripotent Stem Cells"
**作者**: Chen, L. et al.
**摘要**: 利用哺乳动物细胞表达系统制备高纯度DPPA3重组蛋白，实验证明其通过抑制异常甲基化维持iPSCs的基因组稳定性，降低细胞分化风险。
*注：以上文献为示例，实际文献需通过PubMed、Google Scholar等平台检索。*
**Background of DPPA3 Recombinant Protein**
DPPA3 (Developmental Pluripotency-Associated 3), also known as Stella or PGC7. is a protein encoded by the *DPPA3* gene, primarily expressed in germ cells, preimplantation embryos, and pluripotent stem cells. It plays a critical role in early embryonic development and the maintenance of genomic integrity. Structurally, DPPA3 contains a conserved N-terminal helical domain and a C-terminal domain implicated in nucleic acid binding, with a proposed role in regulating epigenetic modifications, such as DNA methylation and chromatin remodeling.
DPPA3 is essential for safeguarding the methylation landscape of imprinted genes and transposable elements during embryogenesis. In oocytes and embryos, it protects maternal DNA from active demethylation, ensuring proper epigenetic reprogramming. Its dysfunction is linked to developmental defects, infertility, and embryonic lethality in model organisms. These features make DPPA3 a key molecule in studying pluripotency, germ cell biology, and epigenetic regulation.
Recombinant DPPA3 protein is produced using expression systems (e.g., *E. coli* or mammalian cells*) to enable functional studies. It is purified via affinity chromatography, often tagged with His or GST for detection. Researchers utilize this protein to investigate its interactions with DNA, histones, or other epigenetic regulators, as well as its role in stem cell differentiation, somatic cell reprogramming, and embryo development.
Beyond basic research, DPPA3 recombinant protein holds potential in reproductive medicine and regenerative therapies. For instance, it may aid in improving the efficiency of induced pluripotent stem cell (iPSC) generation or enhancing the viability of embryos in assisted reproductive technologies. However, its oncogenic potential in certain contexts (e.g., overexpression in cancer cells) necessitates careful exploration. Overall, DPPA3 remains a pivotal tool for unraveling the molecular basis of pluripotency and epigenetic control in development and disease.
×
