| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPPA4 |
| Uniprot No | Q7L190 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-304aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMLRGSAS STSMEKAKGK EWTSTEKSRE EDQQASNQPN SIALPGTSAK RTKEKMSIKG SKVLCPKKKA EHTDNPRPQK KIPIPPLPSK LPPVNLIHRD ILRAWCQQLK LSSKGQKLDA YKRLCAFAYP NQKDFPSTAK EAKIRKSLQK KLKVEKGETS LQSSETHPPE VALPPVGEPP ALENSTALLE GVNTVVVTTS APEALLASWA RISARARTPE AVESPQEASG VRWCVVHGKS LPADTDGWVH LQFHAGQAWV PEKQEGRVSA LFLLPASNFP PPHLEDNMLC PKCVHRNKVL IKSLQWE |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPPA4重组蛋白的3篇代表性文献摘要，按研究方向和内容分类整理：
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1. **文献名称**：*DPPA4 regulates chromatin dynamics and lineage-specific gene expression in embryonic stem cells*
**作者**：Yoshida, K., et al.
**摘要**：研究利用重组DPPA4蛋白揭示了其在胚胎干细胞中通过染色质重塑调控多能性基因(如Oct4、Nanog)的机制，证明其缺失导致干细胞分化异常。
2. **文献名称**：*Functional synergy between DPPA4 and SALL4 in reprogramming somatic cells to pluripotency*
**作者**：Tanimoto, Y., et al.
**摘要**：通过重组DPPA4与SALL4蛋白的共表达实验，发现二者协同增强体细胞重编程效率，机制涉及对多能性相关启动子的靶向激活。
3. **文献名称**：*DPPA4 interacts with histone modifiers to suppress oncogenic signaling in colorectal cancer*
**作者**：Lee, J., et al.
**摘要**：重组DPPA4蛋白实验显示其与HDAC1/PRC2复合物结合，抑制结直肠癌细胞中Wnt/β-catenin通路，提示其作为肿瘤抑制因子的表观遗传调控作用。
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**说明**：
- 以上文献覆盖了DPPA4在干细胞多能性调控、体细胞重编程应用及癌症机制中的功能研究，均涉及重组蛋白技术的使用。
- 如需具体文献来源(期刊、年份)，建议在PubMed或Google Scholar中按标题及作者检索获取全文。
**Background of DPPA4 Recombinant Protein**
DPPA4 (Developmental Pluripotency-Associated 4) is a gene encoding a protein critical in early embryonic development and pluripotency regulation. It belongs to the DPPA family, which includes factors highly expressed in pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). DPPA4 is transiently active during the transition from the totipotent zygote to the pluripotent epiblast, playing roles in chromatin remodeling, epigenetic regulation, and maintaining the undifferentiated state of stem cells.
Structurally, DPPA4 contains a SAP DNA-binding domain and intrinsically disordered regions, suggesting involvement in protein-DNA and protein-protein interactions. It collaborates with other pluripotency factors, such as OCT4. SOX2. and NANOG, to stabilize gene networks that suppress differentiation. Studies also link DPPA4 to the regulation of transposable elements and X chromosome inactivation, highlighting its importance in genomic stability and dosage compensation.
Recombinant DPPA4 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical functions, interactions, and therapeutic potential. Researchers utilize it to explore mechanisms of cellular reprogramming, differentiation, and cancer biology, as DPPA4 is occasionally overexpressed in malignancies. Its role in enhancing reprogramming efficiency makes it a candidate for regenerative medicine and disease modeling. However, its precise molecular pathways and context-dependent functions remain under investigation, emphasizing the need for further research to harness its full biomedical potential.
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