| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHa10 |
| Uniprot No | Q9Y5I2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-948aa |
| 氨基酸序列 | MVSRCSCLGVQCLLLSLLLLAAWEVGSGQLHYSVYEEARHGTFVGRIAQDLGLELAELVQRLFRVASKRHGDLLEVNLQNGILFVNSRIDREELCGRSVECSIHLEVIVDRPLQVFHVDVEVKDINDNPPRFSVTEQKLSIPESRLLDSRFPLEGASDADVGENALLTYKLSPNEYFVLDIINKKDKDKFPVLVLRKLLDREENPQLKLLLTATDGGKPEFTGSVSLLILVLDANDNAPIFDRPVYEVKMYENQVNQTLVIRLNASDSDEGINKEMMYSFSSLVPPTIRRKFWINERTGEIKVNDAIDFEDSNTYEIHVDVTDKGNPPMVGHCTVLVELLDENDNSPEVIVTSLSLPVKEDAQVGTVIALISVSDHDSGANGQVTCSLTPHVPFKLVSTYKNYYSLVLDSALDRERVSAYELVVTARDGGSPPLWATASVSVEVADVNDNAPAFAQSEYTVFVKENNPPGCHIFTVSAWDADAQENALVSYSLVERRLGERSLSSYVSVHAESGKVYALQPLDHEELELLQFQVSARDGGVPPLGSNLTLQVFVLDENDNAPALLASPAGSAGGAVSELVLRSVVAGHVVAKVRAVDADSGYNAWLSYELQSAAVGARIPFRVGLYTGEISTTRALDETDSPRQRLLVLVKDHGEPSLTATATVLVSLVEGSQAPKASSRASVGVAPEVALVDVNVYLIIAICAVSSLLVLTLLLYTALRCSAAPTEGACGPVKPTLVCSSAVGSWSYSQQRRQRVCSGEGLPKADLMAFSPSLPPCPMVDVDGEDQSIGGDHSRKPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ |
| 预测分子量 | 102,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHα10重组蛋白的3篇代表性文献的简要总结(注：因文献检索时效性，以下内容为模拟示例，实际文献需通过数据库查询确认)：
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1. **文献名称**：*Structural characterization of the extracellular domain of PCDHα10 reveals homophilic interaction motifs*
**作者**：Smith J, et al.
**摘要**：本研究通过X射线晶体学解析了重组表达的PCDHα10胞外结构域的三维结构，发现其EC1-EC3结构域中存在独特的β-折叠相互作用界面，揭示了其介导同源细胞黏附的分子机制，为神经突触特异性连接提供结构基础。
2. **文献名称**：*Recombinant PCDHα10 promotes axon guidance in vitro via Wnt signaling pathway*
**作者**：Chen L, et al.
**摘要**：利用HEK293细胞系统表达并纯化重组PCDHα10蛋白，体外实验表明该蛋白通过激活Wnt/β-catenin信号通路促进神经元轴突导向，提示其在神经发育中的潜在调控作用。
3. **文献名称**：*Development of a PCDHα10 recombinant protein-based inhibitor for metastatic cancer cell targeting*
**作者**：Wang Y, et al.
**摘要**：研究团队构建了PCDHα10重组蛋白片段，证明其能特异性结合肿瘤细胞表面受体并抑制转移相关蛋白MMP-9的表达，为开发靶向抗癌药物提供了实验依据。
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如需具体文献，建议通过PubMed或Web of Science以“PCDHA10 recombinant”或“Protocadherin alpha 10 recombinant protein”为关键词检索最新研究。
PCDHα10 (Protocadherin alpha 10) is a member of the clustered protocadherin (PCDH) family, a subgroup of the cadherin superfamily. Protocadherins are transmembrane glycoproteins primarily involved in cell-cell adhesion and signaling, with critical roles in neural development, including neuronal connectivity, synaptic plasticity, and axon guidance. The PCDHα cluster, located on human chromosome 5q31. consists of 15 variable exons (α1–α15) and constant exons. Each variable exon encodes an extracellular domain with six cadherin repeats, a transmembrane domain, and a short cytoplasmic tail. PCDHα10 is one of these variable isoforms, characterized by its unique extracellular domain that mediates homophilic and heterophilic interactions. Its expression is regulated by promoter choice and combinatorial splicing, contributing to neuronal diversity and specificity.
Recombinant PCDHα10 protein is engineered to study its structural and functional properties. Typically produced in mammalian or insect cell systems (e.g., HEK293 or Sf9 cells) to ensure proper post-translational modifications, it retains the extracellular domain responsible for adhesive interactions. The protein often includes a tag (e.g., His-tag or Fc-tag) for purification and detection. Research using recombinant PCDHα10 has highlighted its role in neural circuit assembly, synaptic stabilization, and dendritic self-avoidance. Dysregulation of PCDHα10 has been linked to neurodevelopmental disorders, including autism spectrum disorders and schizophrenia, making it a target for mechanistic and therapeutic studies. Its recombinant form enables in vitro binding assays, structural analysis (e.g., crystallography), and antibody development, advancing insights into cadherin-mediated neural wiring and disease pathology.
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