| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHa4 |
| Uniprot No | Q9UN74 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-947aa |
| 氨基酸序列 | MEFSWGSGQESRRLLLLLLLLAAWEAGNGQLHYSVSEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKGRGGLLEVNLQNGILFVNSRIDREELCRRSAECSIHLEVIVDRPLQVFHVDVEVRDINDNPPVFPATQKNLSIAESRPLDSRFPLEGASDADIGENALLTYRLSPNEYFSLEKPPDDELVKGLGLILRKSLDREEAPEIFLVLTATDGGKPELTGTVQLLITVLDANDNAPAFDRTIYKVRLLENVPNGTLVIKLNASDLDEGLNGDIVYSFSNDISPNVKSKFHIDPITGQIIVKGYIDFEESKSYEIIVEGIDKGQLPLSGHCRVIVEVEDNNDNVPDLEFKSLSLPIREDAPLGTVIALISVSDKDMGVNGLVTCSLTSHVPFKLVSTFKNYYSLVLDSALDRESVSAYELVVTARDGGSPSLWATASVSVEVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSAWDADAQENALVSYSLVERRVGERALSSYVSVHAESGKVYALQPLDHEELELLQFQVTARDAGVPPLGSNVTLQVFVLDENDNAPALLAPRAGGTGGAVSELVPWSVGVGHVVAKVRAVDADSGYNAWLSYELQPGTGGARIPFRVGLYTGEISTTRALDETDAPRHRLLVLVKDHGEPALTATATVLVSLVESGQAPKASSRALVGAVGPDAALVDVNVYLIIAICAVSSLLVLTLLLYTALRCSALPTEGACAPGKPTLVCSSAVGSWSYSQQRRPRVCSGEGPPKTDLMAFSPSLPDSRDREDQLQTTEESFAKPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ |
| 预测分子量 | 102 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHA4重组蛋白的3篇示例参考文献(基于已有研究方向的合理推测，具体文献需以实际检索为准)：
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1. **文献名称**：*Structural characterization of recombinant PCDHA4 extracellular domain and its role in cell-cell adhesion*
**作者**：Smith J, et al.
**摘要**：本研究通过哺乳动物表达系统成功重组表达了PCDHA4的胞外结构域，并利用X射线晶体学解析了其三维结构。实验表明，重组PCDHA4蛋白能够介导同源细胞黏附，并揭示了其钙离子依赖性相互作用机制。
2. **文献名称**：*Recombinant PCDHA4 promotes synaptic density in hippocampal neurons via homophilic binding*
**作者**：Chen L, et al.
**摘要**：作者在大肠杆菌中表达并纯化了功能性PCDHA4重组蛋白，通过体外神经元培养实验发现，该蛋白可特异性结合神经元表面，增强突触形成密度，提示其在神经环路发育中的关键作用。
3. **文献名称**：*Development of a PCDHA4 recombinant protein-based inhibitor for suppressing cancer metastasis*
**作者**：Wang Y, et al.
**摘要**：研究团队利用昆虫细胞系统制备了重组PCDHA4蛋白，发现其能竞争性抑制肿瘤细胞的迁移和侵袭能力，为靶向PCDHA4的癌症治疗策略提供了实验依据。
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**注**：上述文献为示例性内容，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议使用关键词“PCDHA4 recombinant protein”或“protocadherin alpha 4 expression”进一步获取真实文献。
PCDHα4 (Protocadherin alpha 4) is a member of the clustered protocadherin (PCDH) family, a subgroup of calcium-dependent cell adhesion molecules critical for neural development and synaptic specificity. The PCDH gene cluster, located on human chromosome 5q31. comprises three subfamilies (α, β, γ) with diverse extracellular cadherin repeats and variable cytoplasmic domains. PCDHα4. part of the α-subfamily, encodes a single-pass transmembrane protein characterized by six extracellular cadherin domains that mediate homophilic interactions, and a conserved cytoplasmic domain involved in intracellular signaling. Unlike classical cadherins, PCDHs exhibit stochastic monoallelic expression in neurons, contributing to neuronal diversity and circuit assembly.
Recombinant PCDHα4 protein is engineered to study its structural and functional roles in vitro. Produced via mammalian or insect cell expression systems, it retains post-translational modifications essential for calcium-dependent adhesion. Research highlights its involvement in axon guidance, dendritic arborization, and synapse formation, often through interactions with other PCDH isoforms or cytoskeletal regulators like focal adhesion kinases. Dysregulation of PCDHα4 has been linked to neurodevelopmental disorders (e.g., autism, schizophrenia) and cancers (e.g., glioblastoma, lung cancer), where aberrant cell adhesion promotes metastasis or disrupts neural connectivity. Recombinant forms enable biochemical assays, crystallography, and cell-based studies to dissect these mechanisms, offering potential therapeutic targets for precision interventions.
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