| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP12a |
| Uniprot No | P54707 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1039aa |
| 氨基酸序列 | MHQKTPEIYSVELSGTKDIVKTDKGDGKEKYRGLKNNCLELKKKNHKEEFQKELHLDDHKLSNRELEEKYGTDIIMGLSSTRAAELLARDGPNSLTPPKQTPEIVKFLKQMVGGFSILLWVGAFLCWIAYGIQYSSDKSASLNNVYLGCVLGLVVILTGIFAYYQEAKSTNIMSSFNKMIPQQALVIRDSEKKTIPSEQLVVGDIVEVKGGDQIPADIRVLSSQGCRVDNSSLTGESEPQPRSSEFTHENPLETKNICFYSTTCLEGTVTGMVINTGDRTIIGHIASLASGVGNEKTPIAIEIEHFVHIVAGVAVSIGILFFIIAVSLKYQVLDSIIFLIGIIVANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFVADTSEDHSNQVFDQSSRTWASLSKIITLCNRAEFKPGQENVPIMKKAVIGDASETALLKFSEVILGDVMEIRKRNRKVAEIPFNSTNKFQLSIHEMDDPHGKRFLMVMKGAPERILEKCSTIMINGEEHPLDKSTAKTFHTAYMELGGLGERVLGFCHLYLPADEFPETYSFDIDAMNFPTSNLCFVGLLSMIDPPRSTVPDAVTKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANSETVEDIAHRLNIAVEQVNKRDAKAAVVTGMELKDMSSEQLDEILANYQEIVFARTSPQQKLIIVEGCQRQDAVVAVTGDGVNDSPALKKADIGIAMGIAGSDAAKNAADMVLLDDNFASIVTGVEEGRLIFDNLKKTIAYSLTKNIAELCPFLIYIIVGLPLPIGTITILFIDLGTDIIPSIALAYEKAESDIMNRKPRHKNKDRLVNQPLAVYSYLHIGLMQALGAFLVYFTVYAQEGFLPRTLINLRVEWEKDYVNDLKDSYGQEWTRYQREYLEWTGYTAFFVGILVQQIADLIIRKTRRNSIFQQGLFRNKVIWVGITSQIIIGLILSYGLGSVTALSFTMLRAQYWFVAVPHAILIWVYDEVRKLFIRLYPGSWWDKNMYY |
| 预测分子量 | 115 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP12A重组蛋白的3篇参考文献示例(注：文献为虚构示例，仅供格式参考)：
1. **文献名称**：*"Heterologous Expression and Functional Analysis of Recombinant ATP12A in Mammalian Cells"*
**作者**：Zhang, L., et al.
**摘要**：本研究成功在HEK293细胞中表达了重组ATP12A蛋白，验证了其作为H+/K+-ATPase亚基的离子转运活性，并发现其表达水平受细胞外pH调控。
2. **文献名称**：*"Structural Characterization of ATP12A by Cryo-Electron Microscopy Reveals Mechanistic Insights"*
**作者**：Watanabe, K., et al.
**摘要**：通过冷冻电镜解析了重组ATP12A蛋白的高分辨率结构，揭示了其跨膜结构域与ATP水解活性位点的相互作用机制，为靶向药物设计提供了依据。
3. **文献名称**：*"ATP12A Knockout and Recombinant Rescue in Airway Epithelial Cells Highlights Its Role in Mucus Hydration"*
**作者**：Chen, Y., et al.
**摘要**：利用CRISPR敲除气道上皮细胞的ATP12A基因后，通过重组ATP12A蛋白回补实验证实其参与调控细胞膜质子分泌，影响黏液酸碱平衡及黏度。
4. **文献名称**：*"High-Yield Purification of ATP12A from Sf9 Insect Cells for Biochemical Assays"*
**作者**：Müller, R., et al.
**摘要**：开发了一种基于杆状病毒-昆虫细胞系统的重组ATP12A高效纯化方法，获得高纯度蛋白用于体外ATP酶活性及抑制剂筛选研究。
(注：以上文献及作者均为示例，实际研究中请通过PubMed等数据库检索真实文献。)
ATP12A, also known as the human non-gastric H+/K+-ATPase, is a membrane-bound ion transport protein belonging to the P-type ATPase family. It plays a critical role in maintaining cellular pH and ion homeostasis by actively exchanging intracellular H+ for extracellular K+ using energy derived from ATP hydrolysis. Primarily expressed in epithelial tissues (e.g., colon, kidneys, and respiratory tract), ATP12A contributes to acid secretion, mucosal hydration, and electrolyte balance. Dysregulation of ATP12A has been linked to pathologies such as cystic fibrosis (where its hyperactivity exacerbates airway surface acidification) and gastric disorders.
The ATP12A protein consists of two subunits: a catalytic α-subunit (∼1.000 amino acids) containing ATP-binding and phosphorylation domains, and a β-subunit essential for structural stability and membrane trafficking. Recombinant ATP12A proteins are engineered using heterologous expression systems (e.g., mammalian or insect cells) to preserve post-translational modifications and functional integrity. These recombinant variants enable detailed studies of ATP12A’s enzymatic kinetics, ion transport mechanisms, and interactions with inhibitors or therapeutic candidates. Recent research focuses on ATP12A as a potential drug target for acid-related diseases, leveraging recombinant models to screen small molecules or develop biologics that modulate its activity. Its structural elucidation via cryo-EM and mutagenesis studies further advances understanding of P-type ATPase dynamics and evolution.
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