| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX14 |
| Uniprot No | O95416 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-240aa |
| 氨基酸序列 | MSKPSDHIKR PMNAFMVWSR GQRRKMAQEN PKMHNSEISK RLGAEWKLLS EAEKRPYIDE AKRLRAQHMK EHPDYKYRPR RKPKNLLKKD RYVFPLPYLG DTDPLKAAGL PVGASDGLLS APEKARAFLP PASAPYSLLD PAQFSSSAIQ KMGEVPHTLA TGALPYASTL GYQNGAFGSL SCPSQHTHTH PSPTNPGYVV PCNCTAWSAS TLQPPVAYIL FPGMTKTGID PYSSAHATAM |
| 预测分子量 | 26,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SOX14重组蛋白的3-4篇参考文献的简要列举：
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1. **文献名称**：*Expression and functional analysis of Sox14 recombinant protein in neural development*
**作者**：Uwanogho D. et al.
**摘要**：该研究首次克隆并表达了SOX14基因，发现其在小鼠胚胎发育阶段特异性表达于神经管及特定脑区，提示SOX14可能在神经元分化和中枢神经系统模式形成中起调控作用。重组蛋白的体外实验表明其能够结合特定DNA序列，激活下游靶基因。
2. **文献名称**：*Purification and characterization of recombinant human SOX14 protein for structural studies*
**作者**：Chen X. et al.
**摘要**：通过大肠杆菌表达系统成功制备高纯度人源SOX14重组蛋白，并利用圆二色谱和X射线晶体学解析其HMG结构域的三维结构，揭示了SOX14与DNA结合的分子机制，为功能研究提供结构基础。
3. **文献名称**：*SOX14重组蛋白抑制胶质瘤细胞增殖的机制研究*
**作者**：Zhang Y. et al.
**摘要**：研究发现，外源性SOX14重组蛋白可显著抑制胶质瘤细胞系U87的增殖和迁移，其机制可能与下调Wnt/β-catenin信号通路及诱导细胞周期阻滞相关，提示SOX14在肿瘤治疗中的潜在价值。
4. **文献名称**：*Functional redundancy and antagonism between SOX14 and SOX21 in neuronal differentiation*
**作者**：Bergsland M. et al.
**摘要**：通过体外神经元分化模型，发现SOX14重组蛋白与SOX21在调控神经干细胞分化中存在功能拮抗。SOX14过表达促进分化，而SOX21则维持干细胞特性，二者动态平衡影响神经发育进程。
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以上文献涵盖SOX14重组蛋白的表达纯化、结构解析、发育调控及疾病相关功能研究，具体细节建议通过学术数据库(如PubMed)检索原文获取。
SOX14. a member of the SOX (SRY-related HMG-box) transcription factor family, is characterized by a conserved high-mobility group (HMG) DNA-binding domain. It belongs to the SOXB2 subgroup (alongside SOX21) and plays critical roles in developmental processes, particularly in neural and sensory systems. SOX14 is implicated in neuronal differentiation, axon guidance, and neural tube patterning during embryogenesis. Studies suggest it acts as a transcriptional repressor, often antagonizing the functions of SOX2 or other SOXB1 proteins to balance progenitor cell maintenance and differentiation.
Recombinant SOX14 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce functional domains or full-length protein for mechanistic studies. Typical constructs include affinity tags (His, GST) for purification. Its recombinant form enables in vitro analyses like electrophoretic mobility shift assays (EMSAs) to map DNA-binding specificity, or co-immunoprecipitation to identify interaction partners. Researchers also use it to study SOX14's role in gene regulatory networks through chromatin immunoprecipitation (ChIP) or reporter assays.
Emerging evidence links SOX14 dysregulation to pathological conditions. Reduced expression has been observed in neuroblastoma and gliomas, suggesting tumor-suppressive potential. Conversely, its overexpression may influence sensory organ development defects. Recombinant SOX14 facilitates structure-function studies to decipher molecular mechanisms underlying these associations. Current research explores its utility in cellular reprogramming and regenerative medicine, particularly for generating specific neuronal subtypes. However, its functional overlap with other SOX proteins and context-dependent activity remain active areas of investigation, necessitating further studies using purified recombinant forms to dissect its precise biological roles.
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