| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFC1 |
| Uniprot No | P35251 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 402-492aa |
| 氨基酸序列 | GAENCLEGLIFVITGVLESIERDEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKIIDEDGLLNLIRTMPGKKSKYEIA |
| 预测分子量 | 11.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RFC1重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：
1. **文献名称**：*"Recombinant Human RFC1: Expression, Purification, and Functional Analysis in DNA Replication"*
**作者**：Smith J., et al.
**摘要**：该研究报道了人源RFC1重组蛋白在大肠杆菌中的高效表达与纯化方法，证实其与PCNA及RFC复合体其他亚基的相互作用，并验证其在DNA复制中促进DNA聚合酶δ加载的功能。
2. **文献名称**：*"Structural Insights into RFC1’s Role in DNA Damage Repair"*
**作者**：Li X., Wang Y.
**摘要**：通过冷冻电镜解析了RFC1重组蛋白与受损DNA结合的复合物结构，揭示了RFC1在识别DNA损伤位点及招募修复因子中的关键构象变化机制。
3. **文献名称**：*"Functional Characterization of RFC1 Mutants in Mismatch Repair"*
**作者**：Garcia R., et al.
**摘要**：利用重组RFC1蛋白构建多种突变体，发现其ATP酶活性对错配修复过程至关重要，并证明特定结构域突变会导致基因组稳定性下降。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词 **"RFC1 recombinant protein"** 或 **"RFC1 cloning function"** 获取近年研究。
**Background of RFC1 Recombinant Protein**
Replication Factor C subunit 1 (RFC1) is a critical component of the Replication Factor C (RFC) complex, a conserved AAA+ ATPase essential for DNA replication and repair in eukaryotic cells. The RFC complex acts as a clamp loader, facilitating the assembly of Proliferating Cell Nuclear Antigen (PCNA), a sliding clamp protein, onto DNA. This process ensures processive DNA synthesis by DNA polymerases during replication and coordinates repair mechanisms in response to DNA damage.
RFC1. the largest subunit of the RFC complex (120-140 kDa), contains conserved domains for ATP binding/hydrolysis and interaction with other RFC subunits (RFC2-5). Its N-terminal region binds PCNA, while the C-terminal AAA+ domain drives conformational changes required for clamp loading. RFC1 also plays specialized roles in DNA damage checkpoints and telomere maintenance, interacting with proteins like Rad17 and ATAD5.
Recombinant RFC1 is produced using expression systems (e.g., *E. coli* or mammalian cells) for *in vitro* studies. Purified RFC1 enables mechanistic insights into clamp-loader dynamics, replication fidelity, and repair pathways. Mutations in RFC1 are linked to genome instability disorders, making it a target for cancer research. Its recombinant form is vital for reconstituting replication machinery, screening therapeutic agents, and analyzing interactions with PCNA or damaged DNA templates. Structural studies using recombinant RFC1 have advanced understanding of ATP-driven clamp-loading mechanisms and RFC complex regulation.
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