| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Srpx2 |
| Uniprot No | O60687 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-465aa |
| 氨基酸序列 | TWYAGSGYYPDESYNEVYAEEVPQAPALDYRVPRWCYTLNIQDGEATCYSPKGGNYHSSLGTRCELSCDRGFRLIGRRSVQCLPSRRWSGTAYCRQMRCHALPFITSGTYTCTNGVLLDSRCDYSCSSGYHLEGDRSRICMEDGRWSGGEPVCVDIDPPKIRCPHSREKMAEPEKLTARVYWDPPLVKDSADGTITRVTLRGPEPGSHFPEGEHVIRYTAYDRAYNRASCKFIVKVQVRRCPTLKPPQHGYLTCTSAGDNYGATCEYHCDGGYDRQGTPSRVCQSSRQWSGSPPICAPMKINVNVNSAAGLLDQFYEKQRLLIISAPDPSNRYYKMQISMLQQSTCGLDLRHVTIIELVGQPPQEVGRIREQQLSANIIEELRQFQRLTRSYFNMVLIDKQGIDRDRYMEPVTPEEIFTFIDDYLLSNQELTQRREQRDICE |
| 预测分子量 | 57.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SRPX2重组蛋白的示例参考文献(内容基于研究领域常见方向，供参考)：
1. **文献名称**: *SRPX2 enhances angiogenesis through VEGF-VEGFR2 signaling pathway in colorectal cancer*
**作者**: Li, X. et al.
**摘要**: 本研究通过重组SRPX2蛋白处理结肠癌细胞，发现其通过激活VEGF-VEGFR2信号通路促进血管生成，并增强肿瘤微环境中的内皮细胞迁移能力。
2. **文献名称**: *Recombinant SRPX2 improves synaptic plasticity in a mouse model of autism spectrum disorder*
**作者**: Li, H. et al.
**摘要**: 研究利用重组SRPX2蛋白干预自闭症模型小鼠，发现其通过调节突触蛋白PSD-95的表达，改善海马区神经元突触可塑性和认知功能。
3. **文献名称**: *Structural and functional analysis of SRPX2 in cell adhesion*
**作者**: Yamamoto, K. et al.
**摘要**: 通过体外表达纯化的重组SRPX2蛋白，解析其晶体结构，并证明其通过整合素结合域调控细胞-基质黏附，影响胚胎发育中的细胞迁移。
**注**：以上为模拟摘要，实际文献需通过PubMed/Google Scholar检索关键词(如“SRPX2 recombinant protein”)获取。
**Background of Srpx2 Recombinant Protein**
Sushi repeat-containing protein X-linked 2 (Srpx2), also known as SRPUL or MSAP, is a secreted extracellular matrix (ECM)-associated protein belonging to the sushi repeat protein family. It is characterized by multiple sushi-like (or complement control protein) domains, which mediate protein-protein and protein-carbohydrate interactions. Srpx2 is evolutionarily conserved and plays roles in cellular adhesion, tissue development, and signaling regulation.
Studies have linked Srpx2 to critical physiological and pathological processes. It is implicated in synaptogenesis within the central nervous system, where it interacts with neuroligin-1 to promote excitatory synapse formation. Additionally, Srpx2 is involved in cancer progression, angiogenesis, and inflammatory responses. For instance, it binds to urokinase plasminogen activator receptor (uPAR), modulating cell migration and invasion in tumors like glioblastoma. Its dual role—acting as both a tumor promoter and a potential anti-inflammatory mediator—highlights context-dependent functionality.
The recombinant Srpx2 protein is engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., mammalian cells or *E. coli*), it retains biological activity for *in vitro* and *in vivo* studies. Purification typically involves affinity tags (e.g., His-tag) and quality verification via SDS-PAGE or Western blot.
Current research explores Srpx2’s potential as a biomarker or therapeutic target, particularly in neurological disorders and cancers. Its recombinant form enables mechanistic studies, drug screening, and protein interaction analyses, advancing understanding of its multifunctional roles in health and disease.
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