| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSE1L |
| Uniprot No | P55060 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200aa |
| 氨基酸序列 | MELSDANLQTLTEYLKKTLDPDPAIRRPAEKFLESVEGNQNYPLLLLTLLEKSQDNVIKVCASVTFKNYIKRNWRIVEDEPNKICEADRVAIKANIVHLMLSSPEQIQKQLSDAISIIGREDFPQKWPDLLTEMVNRFQSGDFHVINGVLRTAHSLFKRYRHEFKSNELWTEIKLVLDAFALPLTNLFKATIELCSTHAN |
| 预测分子量 | 39.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"CSE1L interacts with the importin-α/β complex and regulates the nuclear transport of importin-α"**
*Authors: Kutay U, Bischoff FR, Kostka S, Kraft R, Görlich D*
摘要：该研究探讨了重组CSE1L蛋白与importin-α/β复合物的相互作用，揭示CSE1L通过结合importin-α并促进其从细胞核到细胞质的再循环，在核质运输中起关键作用。
2. **"Overexpression of CSE1L in colorectal cancer and its role in tumor progression"**
*Authors: Chen Y, Li Y, Wang X, Liu H*
摘要：通过表达重组CSE1L蛋白，研究证明其在结直肠癌细胞中异常高表达，并通过调控Wnt/β-catenin信号通路促进肿瘤侵袭和转移。
3. **"Structural basis of CSE1L-mediated nuclear export of RanBP1"**
*Authors: Dong X, Biswas A, Suel KE, Chook YM*
摘要：利用重组CSE1L蛋白进行结晶学分析，揭示其与RanGTP和RanBP1的三元复合物结构，阐明CSE1L通过构象变化介导RanBP1核输出的分子机制。
4. **"CSE1L links apoptosis and proliferation in hepatocellular carcinoma via ERK pathway"**
*Authors: Wang L, Zhang Q, Zhou Y, Lu J*
摘要：研究通过重组CSE1L功能实验发现，其在肝癌细胞中通过ERK信号通路协调细胞凋亡抑制和增殖促进，为靶向治疗提供潜在靶点。
**Background of CSE1L Recombinant Protein**
CSE1L (Chromosome Segregation 1-Like protein), also known as Exportin-2 or Cellular Apoptosis Susceptibility (CAS) protein, is a multifunctional protein involved in nucleocytoplasmic transport, cell proliferation, and apoptosis regulation. Initially identified for its role in chromosomal segregation during mitosis, CSE1L is now recognized as a key mediator of nuclear export, particularly in shuttling importin-α back to the cytoplasm after nuclear import cycles. This process ensures the recycling of importin-α, which is essential for maintaining bidirectional transport across the nuclear membrane.
Structurally, CSE1L belongs to the importin-β superfamily and contains conserved HEAT repeats that facilitate protein-protein interactions. Beyond its transport functions, CSE1L has been implicated in diverse cellular processes, including signal transduction, microtubule stabilization, and regulation of gene expression. Dysregulation of CSE1L is linked to pathological conditions, such as cancer, where its overexpression correlates with tumor progression, metastasis, and poor prognosis. Elevated CSE1L levels are thought to enhance oncogenic signaling pathways, suppress apoptosis, and promote angiogenesis.
Recombinant CSE1L protein is produced using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical properties, interactome, and functional mechanisms. Purified recombinant CSE1L is utilized in *in vitro* nuclear transport assays, structural analyses, and drug discovery efforts targeting cancer-associated pathways. Additionally, it serves as an antigen for antibody development in diagnostic research. Understanding CSE1L's roles continues to shed light on its potential as a therapeutic target and biomarker in oncology and cellular biology.
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