| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HTR1F |
| Uniprot No | P30939 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-366aa |
| 氨基酸序列 | MDFLNSSDQNLTSEELLNRMPSKILVSLTLSGLALMTTTINSLVIAAIIVTRKLHHPANYLICSLAVTDFLVAVLVMPFSIVYIVRESWIMGQVVCDIWLSVDITCCTCSILHLSAIALDRYRAITDAVEYARKRTPKHAGIMITIVWIISVFISMPPLFWRHQGTSRDDECIIKHDHIVSTIYSTFGAFYIPLALILILYYKIYRAAKTLYHKRQASRIAKEEVNGQVLLESGEKSTKSVSTSYVLEKSLSDPSTDFDKIHSTVRSLRSEFKHEKSWRRQKISGTRERKAATTLGLILGAFVICWLPFFVKELVVNVCDKCKISEEMSNFLAWLGYLNSLINPLIYTIFNEDFKKAFQKLVRCRC |
| 预测分子量 | 41,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HTR1F重组蛋白的3篇参考文献示例(部分为假设性内容，仅供参考)：
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1. **标题**: *"Cloning and Functional Expression of the Human 5-HT1F Receptor Gene in Mammalian Cells"*
**作者**: Johnson R, et al.
**摘要**: 本研究成功克隆了人源HTR1F受体基因，并在HEK293细胞中实现重组表达。通过配体结合实验证实重组蛋白的活性，发现其对5-HT具有高亲和力，为研究该受体在偏头痛中的药理作用奠定基础。
2. **标题**: *"Pharmacological Characterization of Recombinant Human 5-HT1F Receptor: Role in Migraine Therapy"*
**作者**: Smith L, et al.
**摘要**: 利用昆虫细胞(Sf9)系统表达HTR1F重组蛋白，分析其与曲普坦类药物的相互作用。研究表明，重组受体可激活cAMP信号通路，支持HTR1F作为偏头痛治疗的新靶点。
3. **标题**: *"Structural Insights into the 5-HT1F Receptor via Cryo-EM Analysis of the Recombinant Protein Complex"*
**作者**: Chen Z, et al.
**摘要**: 通过冷冻电镜解析HTR1F重组蛋白与其拮抗剂的复合物三维结构，揭示受体跨膜域的独特构象，为设计高选择性抑制剂提供结构基础。
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**备注**：以上文献为示例性质，实际引用时请以真实发表的论文为准(可通过PubMed或Google Scholar搜索关键词“HTR1F recombinant”“5-HT1F expression”获取)。如需具体文献辅助，建议提供更详细的研究方向以便进一步筛选。
**Background of HTR1F Recombinant Protein**
The HTR1F (5-hydroxytryptamine receptor 1F) is a subtype of the serotonin receptor family, a class of G protein-coupled receptors (GPCRs) that mediate the effects of serotonin, a neurotransmitter involved in regulating mood, cognition, and sensory processing. HTR1F is primarily expressed in the central nervous system (CNS), particularly in regions like the cerebral cortex, hippocampus, and thalamus, and has been implicated in neurological and psychiatric conditions, including migraine, anxiety, and depression.
Recombinant HTR1F protein is engineered in vitro using genetic cloning techniques, where the HTR1F gene is inserted into expression systems (e.g., mammalian, insect, or bacterial cells) to produce functional, purified protein for research. This approach ensures high specificity and reproducibility, enabling studies on receptor structure, ligand interactions, and downstream signaling pathways. HTR1F signals through Gαi/o proteins, inhibiting adenylate cyclase and reducing cAMP levels, but may also interact with β-arrestin or other secondary messengers.
Research on HTR1F has gained momentum due to its role in migraine pathophysiology. It is a target for selective agonists like lasmiditan, a migraine-specific drug that bypasses vasoconstrictive effects associated with older serotonin receptor therapies. Recombinant HTR1F facilitates drug screening, mechanism-of-action studies, and structural analyses (e.g., cryo-EM or X-ray crystallography) to design safer therapeutics. Additionally, its expression patterns and signaling crosstalk with other serotonin receptors (e.g., HTR1B/1D) are under investigation to refine CNS-targeted treatments.
Overall, HTR1F recombinant protein serves as a critical tool for unraveling serotonin receptor biology and advancing precision medicine for neurological disorders.
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