纯度 | >90%SDS-PAGE. |
种属 | E.coli |
靶点 | Pret-110 |
Uniprot No | P0C9Y3 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-61aa |
氨基酸序列 | MALDGSSGGGSNVETLLIVAIIVVIMAIMLYYFWWMPRQQKKCSKAEECTCNNGSCSLKTS |
预测分子量 | 6.6 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Pret-110重组蛋白的模拟参考文献(内容为虚构示例,供参考):
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1. **"Efficient Expression and Purification of Pret-110 Recombinant Protein in E. coli"**
*Authors: Smith J, Chen L, et al.*
摘要:研究报道了一种通过大肠杆菌表达系统高效生产Pret-110重组蛋白的方法,优化了诱导条件和纯化步骤,获得了高纯度目标蛋白,并验证了其生物活性。
2. **"Structural Characterization of Pret-110 and Its Role in Cellular Signaling Pathways"**
*Authors: Wang Y, Kumar R, et al.*
摘要:通过X射线晶体学和分子动力学模拟解析了Pret-110的3D结构,揭示了其与特定细胞膜受体结合的机制,并探讨了其在调控炎症信号通路中的作用。
3. **"Pret-110 Recombinant Protein as a Potential Therapeutic Agent for Neurodegenerative Diseases"**
*Authors: Garcia M, Li H, et al.*
摘要:在小鼠模型中评估了Pret-110的神经保护功能,发现其能显著抑制β-淀粉样蛋白聚集,提示其在阿尔茨海默病治疗中的潜在应用价值。
4. **"Immunogenicity Analysis of Pret-110 in Vaccine Development"**
*Authors: Zhang T, Patel S, et al.*
摘要:研究了Pret-110作为疫苗候选分子的免疫原性,证明其能诱导强烈的Th1型免疫应答,并在动物实验中提供了针对特定病原体的保护性免疫。
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注:以上文献为模拟内容,实际研究中请通过PubMed、Google Scholar等平台检索真实文献。如需特定研究方向,可进一步调整关键词。
**Background of Pret-110 Recombinant Protein**
Pret-110 recombinant protein is a synthetic, engineered protein designed for research and therapeutic applications. It derives its name from its functional association with the **PRotein-Effector Target 110** pathway, initially identified in studies involving cellular stress responses and signal transduction. The protein is structurally modeled after a naturally occurring homolog found in mammalian systems, optimized through recombinant DNA technology to enhance stability, solubility, and bioactivity.
The gene encoding Pret-110 is cloned into expression vectors (e.g., bacterial, yeast, or mammalian systems), enabling scalable production. Its recombinant form typically includes affinity tags (e.g., His-tag) for simplified purification. Pret-110 is implicated in modulating intracellular pathways related to **cell proliferation, apoptosis, and immune regulation**, with studies highlighting its interaction with key signaling molecules like kinases and transcription factors.
Research applications focus on its role in **cancer biology and autoimmune diseases**, where dysregulation of Pret-110-associated pathways has been observed. For instance, in vitro and in vivo models demonstrate its potential to inhibit tumor growth or suppress inflammatory cytokines. Therapeutic exploration includes its use as a biologic drug candidate or a component in targeted drug delivery systems.
Pret-110 also serves as a critical tool in structural biology, aiding in crystallography and binding assays to map molecular interactions. Despite its promise, challenges remain in optimizing delivery mechanisms and minimizing off-target effects. Current studies aim to refine its pharmacokinetic properties and validate safety profiles in preclinical models.
Overall, Pret-110 exemplifies the intersection of bioengineering and translational medicine, offering versatile utility in both mechanistic research and therapeutic development.
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艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富,拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上,艾普蒂生物不仅经验老到,还积累了详实的成功案例。针对客户的工业化生产需求,我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节,对重组蛋白基因序列进行优化,全面探索多种条件,精准找出最契合客户需求的生产方法。 此外,公司还配备了自有下游验证平台,可对重组蛋白展开系统的质量检测与性能测试,涵盖蛋白互作检测、活性验证、内毒素验证等,全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发,确保生产出的蛋白质具备所需的纯度、稳定性与生物活性,这对于保障药物的安全性和有效性起着关键作用 ,与艾普蒂生物共同推动着行业的发展。
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