| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CFDP1 |
| Uniprot No | Q9UEE9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 氨基酸序列 | MEEFDSEDFSTSEEDEDYVPSGGEYSEDDVNELVKEDEVDGEEQTQKTQGKKRKAQSIPARKRRQGGLSLEEEEEEDANSESEGSSSEEEDDAAEQEKGIGSEDARKKKEDELWASFLNDVGPKSKVPPSTQVKKGEETEETSSSKLLVKAEELEKPKETEKVKITKVFDFAGEEVRVTKEVDATSKEAKSFFKQNEKEKPQANVPSALPSLPAGSGLKRSSGMSSLLGKIGAKKQKMSTLEKSKLDWESFKEEEGIGEELAIHNRGKEGYIERKAFLDRVDHRQFEIERDLRLSKMKP |
| 预测分子量 | 60.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于研究领域常见结构的模拟参考文献示例(实际文献请通过学术数据库核实)：
1. **《Recombinant CFDP1 Protein Expression and Purification in E. coli》**
- 作者：Zhang, L. et al.
- 摘要：本研究利用大肠杆菌系统成功表达并纯化了CFDP1重组蛋白，通过His标签亲和层析和分子筛色谱获得高纯度蛋白，为后续功能研究提供材料基础。
2. **《Structural Characterization of CFDP1 and Its Role in Chromatin Remodeling》**
- 作者：Wang, Y. & Smith, J.
- 摘要：通过X射线晶体学解析了CFDP1重组蛋白的三维结构，发现其与染色质重塑复合物相互作用，可能参与调控基因转录和胚胎发育过程。
3. **《CFDP1 Recombinant Protein Inhibits Tumor Growth in a Mouse Xenograft Model》**
- 作者：Chen, H. et al.
- 摘要：在哺乳动物细胞中表达CFDP1重组蛋白，体内实验表明其通过诱导细胞周期阻滞抑制肿瘤生长，提示其潜在抗癌治疗价值。
4. **《Functional Analysis of CFDP1 in Ciliogenesis Using Recombinant Protein Rescue Assays》**
- 作者：Garcia, R. et al.
- 摘要：通过重组CFDP1蛋白回补实验，证实其在纤毛形成中的关键作用，并揭示其与CEP290蛋白的相互作用机制。
**注意事项**：以上内容为示例性模拟，实际文献需通过PubMed、Web of Science或Google Scholar等平台检索关键词(如“CFDP1 recombinant protein”“Craniofacial development protein 1”)获取。建议结合具体研究方向筛选实验设计或疾病模型相关的高影响力论文。
**Background of CFDP1 Recombinant Protein**
The Craniofacial Development Protein 1 (CFDP1), also known as Bucentaur (BCNT), is a conserved eukaryotic protein implicated in transcriptional regulation, chromatin remodeling, and embryonic development. The human *CFDP1* gene, located on chromosome 16q23.1. encodes a protein containing a SYF2 homology domain and a C-terminal BCNT domain, both critical for its interactions with chromatin-modifying complexes and nucleic acids. CFDP1 plays a role in craniofacial morphogenesis, as evidenced by studies linking its dysfunction to developmental anomalies in model organisms.
Recombinant CFDP1 protein is engineered to enable functional and structural studies. It is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography (e.g., His-tag). The recombinant protein retains biological activity, allowing researchers to investigate its interactions with DNA, histones, or partner proteins (e.g., components of the SSU72 phosphatase complex) in vitro.
Research on CFDP1 has expanded into its potential roles beyond development, including DNA repair and cell cycle regulation. Dysregulation of CFDP1 has been associated with cancers, such as hepatocellular carcinoma, where it may act as a tumor suppressor. Additionally, its conserved BCNT domain suggests evolutionary significance in chromatin dynamics across species.
The availability of recombinant CFDP1 facilitates mechanistic studies, drug screening, and antibody production, contributing to understanding its physiological and pathological relevance. Ongoing research aims to clarify its precise molecular mechanisms and therapeutic potential in developmental disorders and cancer.
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