| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENPK |
| Uniprot No | Q9BS16 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-269aa |
| 氨基酸序列 | MNQEDLDPDSTTDVGDVTNTEEELIRECEEMWKDMEECQNKLSLIGTETLTDSNAQLSLLIMQVKCLTAELSQWQKKTPETIPLTEDVLITLGKEEFQKLRQDLEMVLSTKESKNEKLKEDLEREQRWLDEQQQIMESLNVLHSELKNKVETFSESRIFNELKTKMLNIKEYKEKLLSTLGEFLEDHFPLPDRSVKKKKKNIQESSVNLITLHEMLEILINRLFDVPHDPYVKISDSFWPPYVELLLRNGIALRHPEDPTRIRLEAFHQ |
| 预测分子量 | 58.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CENPK重组蛋白的3篇代表性文献示例(内容基于真实研究领域，但文献标题和作者为虚拟示例)：
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1. **标题**：*Structural and Functional Analysis of Human CENPK in Centromere Assembly*
**作者**：Smith J, Lee T, et al.
**摘要**：本研究通过重组表达纯化了人源CENPK蛋白，解析了其N端结构域的晶体结构，发现其通过与CENP-C和CENP-N相互作用参与着丝粒复合体组装，为染色体分离机制提供了新见解。
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2. **标题**：*CENPK Knockdown and Recombinant Rescue in Mitotic Fidelity*
**作者**：Zhang Y, Wang H, et al.
**摘要**：利用siRNA敲低CENPK导致染色体错配，通过重组CENPK蛋白回补实验证实其通过调控动粒-微管连接维持有丝分裂准确性，提示其在癌症基因组不稳定性中的潜在作用。
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3. **标题**：*Recombinant CENPK Expression in a Prokaryotic System for Antibody Production*
**作者**：Kumar R, Gupta S.
**摘要**：优化了CENPK在大肠杆菌中的重组表达条件，纯化后免疫小鼠获得高特异性多克隆抗体，验证了其在免疫荧光和Western blot中的应用，为临床检测提供工具。
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**注**：以上文献为示例性内容，实际研究中建议通过PubMed或Google Scholar以关键词“CENPK recombinant”检索最新论文。如需真实文献，可提供具体研究方向进一步筛选。
CENPK (Centromere Protein K) is a critical component of the centromere complex, playing a pivotal role in ensuring accurate chromosome segregation during cell division. It is part of the constitutive centromere-associated network (CCAN), which stabilizes the kinetochore-microtubule interface and facilitates proper spindle attachment. Dysregulation of CENPK has been linked to chromosomal instability, a hallmark of various cancers and genetic disorders.
Recombinant CENPK protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to produce purified, functional protein for research. This involves cloning the *CENPK* gene into expression vectors, followed by transfection, protein extraction, and affinity chromatography-based purification (e.g., His-tag systems). The recombinant form retains biological activity, enabling studies on its structure, interactions, and role in centromere assembly.
Research applications include *in vitro* binding assays to map centromere protein networks, enzymatic studies to explore post-translational modifications (e.g., phosphorylation), and cellular assays to assess mitotic defects upon CENPK depletion or mutation. It also serves as an antigen for antibody development. Notably, CENPK overexpression in cancers correlates with poor prognosis, making it a potential therapeutic target. Recombinant CENPK aids in drug screening to identify inhibitors disrupting centromere function in malignant cells.
Despite progress, challenges remain in understanding its dynamic regulation during the cell cycle and tissue-specific roles. Ongoing studies aim to resolve its 3D structure and explore its interplay with other centromeric components, advancing insights into mitotic mechanisms and cancer biology.
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