| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HYP2 |
| Uniprot No | P23301 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-157aa |
| 氨基酸序列 | SDEEHTFETADAGSSATYPMQCSALRKNGFVVIKSRPCKIVDMSTSKTGKHGHAKVHLVAIDIFTGKKLEDLSPSTHNMEVPVVKRNEYQLLDIDDGFLSLMNMDGDTKDDVKAPEGELGDSLQTAFDEGKDLMVTIISAMGEEAAISFKEAARTD |
| 预测分子量 | 33.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HYP2重组蛋白的模拟参考文献示例(文献为虚构，仅供格式参考)：
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1. **文献名称**：*Recombinant HYP2 Facilitates Stress Granule Assembly in Saccharomyces cerevisiae*
**作者**：Zhang, L.; Chen, M.; Wang, Y.
**摘要**：本研究通过在大肠杆菌中表达并纯化重组HYP2蛋白，探究其在酵母应激颗粒形成中的作用。结果显示，HYP2通过结合特定RNA序列促进应激颗粒的聚集，且在热应激条件下显著增强细胞存活率。
2. **文献名称**：*Structural Characterization of HYP2 Recombinant Protein Using Cryo-EM*
**作者**：Johnson, R.; Smith, K.; Lee, J.
**摘要**：利用冷冻电镜技术解析了重组HYP2蛋白的三维结构，揭示了其N端结构域在催化活性中的关键作用，为设计靶向HYP2的小分子抑制剂提供了结构基础。
3. **文献名称**：*Optimization of HYP2 Expression in Pichia pastoris for Industrial Applications*
**作者**：Patel, S.; Gupta, A.; Kim, H.
**摘要**：通过优化启动子系统和发酵条件，在毕赤酵母中实现了重组HYP2的高效表达，产量较传统系统提升3倍，为大规模生产应用于生物催化奠定了基础。
4. **文献名称**：*HYP2 Knockout Reveals Its Role in Translational Regulation under Hypoxia*
**作者**：Brown, T.; Müller, F.; Ito, T.
**摘要**：构建HYP2基因敲除酵母株，发现HYP2缺失导致缺氧条件下蛋白质翻译效率下降，表明其在调控核糖体功能中起重要作用。
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**提示**：实际文献需通过PubMed、Google Scholar等平台检索关键词(如“HYP2 recombinant protein”“yeast Hyp2 function”)。建议结合具体研究领域(如结构生物学、细胞应激响应等)筛选文献。
**Background of HYP2 Recombinant Protein**
HYP2 recombinant protein is a genetically engineered biomolecule derived from the human HYAL2 (hyaluronidase-2) gene, which encodes a lysosomal enzyme involved in the degradation of hyaluronic acid (HA), a major component of the extracellular matrix. HYAL2 is part of the hyaluronidase family, playing a critical role in regulating HA turnover, cell signaling, and tissue remodeling. Unlike other hyaluronidases, HYAL2 exhibits limited enzymatic activity at neutral pH, functioning primarily in acidic environments such as lysosomes or tumor microenvironments.
The recombinant form of HYAL2 (HYP2) is typically produced using expression systems like *E. coli* or mammalian cell cultures, enabling large-scale production with high purity and consistency. Its structure often includes post-translational modifications to mimic native functionality, such as glycosylation sites critical for substrate binding. HYP2 has garnered attention in biomedical research due to its dual role in both HA degradation and cellular processes like apoptosis, proliferation, and cancer metastasis. Studies suggest HYAL2 overexpression in certain cancers correlates with tumor aggressiveness and poor prognosis, positioning it as a potential therapeutic target or diagnostic biomarker.
In drug development, HYP2 recombinant protein is utilized to study HA-mediated pathways, screen inhibitors, or engineer targeted therapies. It also serves as a tool in tissue engineering to modulate HA-rich matrices for regenerative applications. Despite its promise, challenges remain in fully elucidating its mechanistic diversity across tissues and disease contexts. Ongoing research aims to clarify its interactions with HA fragments and signaling receptors, such as LYVE-1 and CD44. to unlock therapeutic potential in oncology, fibrosis, and inflammatory diseases.
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