| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF2F2 |
| Uniprot No | P13984 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-249aa |
| 氨基酸序列 | AERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEVSFTLNEDLANIHDIGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAASENYMRLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEKSD |
| 预测分子量 | 44.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与GTF2F2重组蛋白相关的3篇文献示例(内容基于领域内常见研究方向综合概括)：
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1. **文献名称**：**"Structural insights into the assembly and function of the RNA polymerase II transcription machinery"**
**作者**：Cramer, P., et al.
**摘要**：该研究通过重组表达并纯化人源RNA聚合酶II及其相关转录因子(包括GTF2F2)，解析了转录起始复合物的冷冻电镜结构，揭示了GTF2F2在稳定RNA聚合酶II与启动子结合中的关键作用，为理解真核生物转录机制提供了结构基础。
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2. **文献名称**：**"Recombinant expression and functional characterization of human GTF2F2 in transcriptional regulation"**
**作者**：Tan, S., et al.
**摘要**：研究者通过大肠杆菌系统成功表达并纯化重组GTF2F2蛋白，结合体外转录实验证明其与TFIIF复合体另一亚基(GTF2F1)的相互作用对RNA聚合酶II的转录活性至关重要，并揭示了其参与启动子逃逸的分子机制。
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3. **文献名称**：**"Dynamic interactions of GTF2F2 with RNA polymerase II during transcription initiation and elongation"**
**作者**：Conaway, R.C., & Conaway, J.W.
**摘要**：通过重组蛋白技术构建GTF2F2突变体，研究其在不同转录阶段(起始与延伸)的动态结合模式，发现GTF2F2通过构象变化调控RNA聚合酶II的磷酸化状态，影响转录进程的保真性和效率。
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**备注**：以上文献为领域典型研究方向概括，实际引用时建议通过PubMed或Web of Science检索具体论文(关键词：GTF2F2 recombinant protein, TFIIF complex, RNA polymerase II transcription)。如需实验方案，可参考蛋白质表达纯化类文献(如《Nature Protocols》相关方法学论文)。
**Background of GTF2F2 Recombinant Protein**
GTF2F2 (General Transcription Factor IIF subunit 2) is a critical component of the RNA polymerase II transcription machinery, playing a central role in the regulation of gene expression. It forms a heterodimer with GTF2F1 to create the transcription factor IIF (TFIIF), which is essential for transcription initiation and elongation. TFIIF stabilizes the binding of RNA polymerase II to promoter regions, facilitates promoter clearance, and enhances transcriptional fidelity by coordinating with other general transcription factors (e.g., TFIIB, TFIID) and mediator complexes.
The recombinant GTF2F2 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its production involves inserting the GTF2F2 gene into expression vectors, followed by purification via affinity chromatography. This recombinant form retains the functional properties of the native protein, enabling *in vitro* studies on transcription mechanisms, protein-protein interactions, and regulatory networks.
Research applications of GTF2F2 recombinant protein include investigating its role in diseases linked to transcriptional dysregulation, such as cancers and developmental disorders. Mutations or altered expression of GTF2F2 have been implicated in impaired cellular proliferation and differentiation, highlighting its therapeutic potential. Additionally, it serves as a tool for drug screening aimed at modulating transcriptional activity.
In summary, GTF2F2 recombinant protein is a vital resource for dissecting the molecular basis of transcription and exploring interventions for transcription-related pathologies. Its study bridges fundamental biochemistry with translational biomedical research.
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