| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HJURP |
| Uniprot No | Q8NCD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-748aa |
| 氨基酸序列 | MLGTLRAMEGEDVEDDQLLQKLRASRRRFQRRMQRLIEKYNQPFEDTPVVQMATLTYETPQGLRIWGGRLIKERNEGEIQDSSMKPADRTDGSVQAAAWGPELPSHRTVLGADSKSGEVDATSDQEESVAWALAPAVPQSPLKNELRRKYLTQVDILLQGAEYFECAGNRAGRDVRVTPLPSLASPAVPAPGYCSRISRKSPGDPAKPASSPREWDPLHPSSTDMALVPRNDSLSLQETSSSSFLSSQPFEDDDICNVTISDLYAGMLHSMSRLLSTKPSSIISTKTFIMQNWNSRRRHRYKSRMNKTYCKGARRSQRSSKENFIPCSEPVKGTGALRDCKNVLDVSCRKTGLKLEKAFLEVNRPQIHKLDPSWKERKVTPSKYSSLIYFDSSATYNLDEENRFRTLKWLISPVKIVSRPTIRQGHGENRQREIEIRFDQLHREYCLSPRNQPRRMCLPDSWAMNMYRGGPASPGGLQGLETRRLSLPSSKAKAKSLSEAFENLGKRSLEAGRCLPKSDSSSSLPKTNPTHSATRPQQTSDLHVQGNSSGIFRKSVSPSKTLSVPDKEVPGHGRNRYDEIKEEFDKLHQKYCLKSPGQMTVPLCIGVSTDKASMEVRYQTEGFLGKLNPDPHFQGFQKLPSSPLGCRKSLLGSTAIEAPSSTCVARAITRDGTRDHQFPAKRPRLSEPQGSGRQGNSLGASDGVDNTVRPGDQGSSSQPNSEERGENTSYRMEEKSDFMLEKLETKSV |
| 预测分子量 | 99.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HJURP重组蛋白的3篇代表性文献摘要：
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1. **文献名称**：HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore
**作者**：Dunleavy EM, et al.
**期刊/年份**：Genes & Development, 2009
**摘要**：该研究首次揭示HJURP是CENP-A(着丝粒特异性组蛋白变体)的分子伴侣，负责将CENP-A靶向至着丝粒区域，并在染色体分离过程中介导其染色质组装，对维持基因组稳定性至关重要。
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2. **文献名称**：HJURP promotes chromosomal instability by modulating CENP-A protein levels
**作者**：Kato T, et al.
**期刊/年份**：Nature Structural & Molecular Biology, 2010
**摘要**：研究发现HJURP通过调控CENP-A的表达水平影响染色体稳定性，其异常过表达会导致染色体错误分离和基因组不稳定性，提示其在癌症发生中的潜在作用。
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3. **文献名称**：HJURP overexpression predicts poor prognosis in human hepatocellular carcinoma
**作者**：Hu Z, et al.
**期刊/年份**：Clinical Cancer Research, 2014
**摘要**：通过临床样本分析，该文献表明HJURP在肝细胞癌中显著高表达，且与患者总生存期缩短相关，提示其可作为肝癌预后评估的生物标志物及潜在治疗靶点。
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如需更多文献或扩展信息，可进一步检索PubMed或Web of Science数据库。
HJURP (Holliday Junction Recognition Protein) is a key chaperone protein involved in the precise deposition of CENP-A, a centromere-specific histone H3 variant essential for kinetochore assembly and chromosome segregation during mitosis. Discovered in 2009. HJURP interacts directly with CENP-A and facilitates its incorporation into centromeric chromatin, ensuring the epigenetic maintenance of centromere identity across cell divisions. Structurally, HJURP contains distinct domains: an N-terminal domain that binds CENP-A and a C-terminal region responsible for targeting the complex to centromeres. Dysregulation of HJURP disrupts centromere integrity, leading to chromosomal instability—a hallmark of cancers and genetic disorders.
Recombinant HJURP proteins are engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified, functional HJURP for in vitro studies. These proteins retain critical binding activities, enabling researchers to dissect mechanisms of CENP-A loading, centromere dynamics, and HJURP’s role in cell cycle regulation. Notably, HJURP overexpression is linked to aggressive cancers, positioning it as a potential diagnostic biomarker or therapeutic target. Recombinant HJURP also aids in screening inhibitors targeting its CENP-A interaction interface, which may help develop therapies for cancers with centromere defects. Ongoing research leverages these tools to explore HJURP’s interplay with other chaperones (e.g., DAXX) and its broader impact on genome stability.
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