| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Hist1h2ba |
| Uniprot No | P70696 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-127aa |
| 氨基酸序列 | PEVAVKGATISKKGFKKAVTKTQKKEGRKRKRCRKESYSIYIYKVLKQVHPDTGISSKAMSIMNSFVTDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK |
| 预测分子量 | 21.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Hist1h2ba重组蛋白的示例参考文献(注：以下内容为假设性示例，实际文献需通过学术数据库检索确认)：
1. **文献名称**: "Expression and purification of recombinant Hist1h2ba for chromatin assembly studies"
**作者**: Smith A, et al.
**摘要**: 本研究描述了通过大肠杆菌表达系统高效表达并纯化重组Hist1h2ba蛋白的方法，验证了其与组蛋白H2A形成异源二聚体的能力，为体外染色质重建实验提供了关键材料。
2. **文献名称**: "Hist1h2ba post-translational modifications regulate DNA damage response"
**作者**: Lee JH, et al.
**摘要**: 利用重组Hist1h2ba蛋白探究其磷酸化修饰(如Ser37位点)在DNA损伤修复中的作用，发现修饰后的蛋白增强了染色质松散化，促进修复因子募集。
3. **文献名称**: "Structural insights into Hist1h2ba-nucleosome interactions by cryo-EM"
**作者**: Zhang Y, et al.
**摘要**: 通过重组Hist1h2ba与其它核心组蛋白共表达组装核小体，结合冷冻电镜解析其三维结构，揭示了该亚型特异性参与染色质高级结构调控的分子机制。
4. **文献名称**: "Hist1h2ba recombinant protein-based screening for epigenetic inhibitors"
**作者**: Tanaka K, et al.
**摘要**: 开发基于重组Hist1h2ba的体外甲基化检测体系，用于高通量筛选靶向组蛋白修饰酶的小分子抑制剂，发现两种化合物可显著抑制H2B相关甲基转移酶活性。
**提示**：实际研究中建议通过PubMed、Web of Science等平台以关键词"Hist1h2ba recombinant"或"recombinant H2B histone"检索最新文献，重点关注蛋白表达、功能分析或结构研究相关论文。
**Background of Hist1h2ba Recombinant Protein**
Hist1h2ba, a member of the histone H2B family, is a core component of nucleosomes, the fundamental repeating units of chromatin. Histones play a critical role in packaging DNA into structural hierarchies, regulating gene expression, and maintaining genome stability. As a subtype of H2B, Hist1h2ba is encoded by the *Hist1* gene cluster, which houses multiple histone genes expressed primarily during the S phase of the cell cycle.
Structurally, Hist1h2ba shares the conserved histone fold domain common to all H2B variants, facilitating its interaction with H2A, H3. and H4 to form the octameric core around which DNA wraps. Its N-terminal tail undergoes post-translational modifications (e.g., acetylation, phosphorylation, ubiquitination), which influence chromatin remodeling, transcriptional regulation, and DNA repair. Unlike some H2B variants with tissue-specific roles, Hist1h2ba is ubiquitously expressed, suggesting a broad functional relevance in cellular processes.
Recombinant Hist1h2ba protein is typically produced using heterologous expression systems (e.g., *E. coli* or yeast), enabling high-purity yields for biochemical and functional studies. Its recombinant form retains the ability to assemble into nucleosomes, making it valuable for *in vitro* studies on chromatin dynamics, epigenetic mechanisms, and interactions with DNA or regulatory proteins. Researchers also utilize it to investigate how specific mutations or post-translational modifications alter nucleosome stability or gene regulatory pathways.
Dysregulation of histone H2B variants, including Hist1h2ba, has been linked to diseases such as cancer, where aberrant chromatin states drive oncogenesis. Thus, recombinant Hist1h2ba serves as a tool to explore therapeutic targets in epigenetics. Its study continues to shed light on the intricate balance between chromatin structure and cellular function.
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