| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HAND2 |
| Uniprot No | P61296 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-180aa |
| 氨基酸序列 | MSLVGGFPHHPVVHHEGYPFAAAAAASRCSHEENPYFHGWLIGHPEMSPPDYSMALSYSPEYASGTANRKERRRTQSINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDLLAKDDQNGEAEAFKAEIKKTDVKEEKRKKELNEILKSTVSSNDKKTKGRTGWPQHVWALELKQ |
| 预测分子量 | 47.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HAND2重组蛋白的模拟参考文献示例(注：实际文献需通过学术数据库查询)：
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1. **文献名称**：*HAND2 Recombinant Protein Promotes Cardiomyocyte Differentiation in Embryonic Stem Cells*
**作者**：Smith J, Lee A, et al.
**摘要**：研究利用重组HAND2蛋白处理小鼠胚胎干细胞，发现其通过激活心脏发育相关基因(如Nkx2.5和GATA4)，显著增强心肌细胞分化效率，为心脏再生治疗提供新策略。
2. **文献名称**：*Structural and Functional Analysis of HAND2 Recombinant Protein in Limb Development*
**作者**：Garcia R, Patel M, et al.
**摘要**：通过体外表达纯化HAND2重组蛋白，揭示其与DNA结合域的关键氨基酸位点，并证明其在鸡胚肢体发育模型中调控Shh信号通路，影响肢体模式形成。
3. **文献名称**：*HAND2 Overexpression via Recombinant Protein Delivery Attenuates Heart Failure in a Rodent Model*
**作者**：Chen L, Wang Y, et al.
**摘要**：将重组HAND2蛋白注射至心肌梗死大鼠模型，发现其通过抑制纤维化和促进血管生成改善心脏功能，表明HAND2蛋白在心力衰竭治疗中的潜在应用。
4. **文献名称**：*Epigenetic Regulation by HAND2 Recombinant Protein in Neural Crest Cell Migration*
**作者**：Kim S, Tanaka K, et al.
**摘要**：研究证明重组HAND2蛋白通过调控神经嵴细胞中Snail1和Twist1的表达，影响细胞迁移和分化，为颅面发育异常疾病机制提供新见解。
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**建议**：以上为模拟示例，实际文献可通过PubMed、Google Scholar等平台以关键词“HAND2 recombinant protein”或“HAND2 protein function”检索，并筛选近年高影响力研究。
**Background of HAND2 Recombinant Protein**
HAND2 (heart- and neural crest derivatives-expressed protein 2) is a member of the basic helix-loop-helix (bHLH) transcription factor family, playing pivotal roles in embryonic development, particularly in cardiogenesis, limb formation, and craniofacial morphogenesis. It is essential for the differentiation and maturation of cardiac cells, including the right ventricle and outflow tract, and interacts with signaling pathways like FGF, BMP, and retinoic acid to regulate tissue-specific gene expression.
Recombinant HAND2 protein is engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. This allows precise study of its mechanisms in development and disease. Applications include investigating congenital defects (e.g., heart malformations, limb abnormalities) and exploring its role in cancer, where HAND2 dysregulation is linked to tumor progression or suppression.
In regenerative medicine, HAND2 recombinant protein is explored for tissue engineering, such as promoting cardiomyocyte differentiation in stem cell-based therapies. Its ability to modulate gene networks makes it a valuable tool for developmental biology and disease modeling. Challenges remain in optimizing delivery methods and understanding context-dependent interactions in different tissues. Overall, HAND2 recombinant protein serves as a critical resource for deciphering developmental pathways and advancing therapeutic strategies.
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