| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H2BC3 |
| Uniprot No | P33778 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-126aa |
| 氨基酸序列 | PEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK |
| 预测分子量 | 29.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于H2BC3重组蛋白的3篇参考文献示例(注：以下内容为模拟创作，仅供参考)：
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1. **文献名称**: "Expression and Purification of Recombinant H2BC3 in Escherichia coli for Epigenetic Studies"
**作者**: Smith A, et al.
**摘要**: 本研究描述了一种高效表达和纯化重组H2BC3蛋白的方法，利用大肠杆菌系统，并通过质谱验证其正确修饰，为后续染色质相互作用研究提供工具。
2. **文献名称**: "H2BC3 Recombinant Protein Facilitates Nucleosome Assembly in Vitro"
**作者**: Tanaka K, et al.
**摘要**: 通过体外重构实验，证明重组H2BC3与组蛋白伴侣蛋白协作参与核小体组装，揭示了其在染色质动态调控中的潜在作用。
3. **文献名称**: "Structural Analysis of H2BC3 Post-Translational Modifications Using Recombinant Protein"
**作者**: Lee J, et al.
**摘要**: 利用重组H2BC3结合质谱技术，系统鉴定了其在赖氨酸位点的乙酰化和泛素化修饰，为表观遗传机制研究提供新见解。
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**注**：若需真实文献，建议通过PubMed或Google Scholar搜索关键词“H2BC3 recombinant protein”或“H2B histone recombinant expression”获取最新研究。
H2BC3 (Histone H2B Clustered Member 3) is a subtype of the H2B histone family, which plays a fundamental role in organizing eukaryotic DNA into chromatin. As a core component of nucleosomes, H2B histones interact with H2A, H3. and H4 histones to form an octamer around which DNA is wrapped. This structural arrangement regulates DNA accessibility, influencing gene expression, replication, and repair. The H2BC3 gene, located on chromosome 6 in humans, encodes a replication-dependent histone that is critical during cell division and differentiation.
Recombinant H2BC3 protein is produced using genetic engineering techniques, typically by cloning the H2BC3 gene into expression vectors (e.g., bacterial or mammalian systems) followed by purification via affinity chromatography. This engineered protein retains the biochemical properties of native H2B histones, including post-translational modification sites (e.g., acetylation, phosphorylation) that modulate chromatin dynamics. Researchers utilize recombinant H2BC3 to study epigenetic mechanisms, nucleosome assembly, and DNA-protein interactions in vitro. It also serves as a tool for investigating histone-mediated processes in diseases such as cancer, where aberrant histone modifications are linked to transcriptional dysregulation.
Recent studies highlight its potential in exploring histone exchange dynamics and crosstalk with other epigenetic regulators. Challenges in production include maintaining proper folding and post-translational modifications, often requiring eukaryotic expression systems. Overall, recombinant H2BC3 is a vital reagent for advancing chromatin biology and therapeutic strategies targeting epigenetic disorders.
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