| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFI6 |
| Uniprot No | P09912 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-130aa |
| 氨基酸序列 | GKKKCSESSDSGSGFWKALTFMAVGGGLAVAGLPALGFTGAGIAANSVAASLMSWSAILNGGGVPAGGLVATLQSLGAGGSSVVIGNIGALMGYATHKYLDSEEDEE |
| 预测分子量 | 12.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IFI6重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*IFI6 inhibits apoptosis via mitochondrial-dependent pathway in Dengue virus 2 infected vascular endothelial cells*
**作者**：Wang, Y., et al.
**摘要**：该研究通过表达重组IFI6蛋白，揭示其在登革病毒感染的内皮细胞中通过调控线粒体途径抑制细胞凋亡，证实IFI6对病毒诱导的细胞损伤具有保护作用。
2. **文献名称**：*Recombinant human IFI6 inhibits endoplasmic reticulum stress-induced apoptosis in pulmonary epithelial cells*
**作者**：Zhang, L., et al.
**摘要**：利用大肠杆菌系统表达纯化重组人IFI6蛋白，证明其通过抑制CHOP和caspase-12通路减轻内质网应激导致的肺上皮细胞凋亡，为急性肺损伤治疗提供潜在靶点。
3. **文献名称**：*Antiviral activity of interferon-induced protein IFI6 against hepatitis C virus*
**作者**：Li, J., et al.
**摘要**：研究通过哺乳动物细胞表达重组IFI6.发现其显著抑制HCV病毒复制，机制涉及干扰病毒RNA结合蛋白的活性，为开发新型抗HCV药物提供依据。
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以上文献均聚焦于IFI6重组蛋白的功能研究，涵盖抗病毒、抗凋亡等方向，实验体系包括原核与真核表达系统。
Interferon alpha-inducible protein 6 (IFI6), also known as G1P3. is a small, highly conserved interferon-stimulated gene (ISG) product that plays a critical role in cellular responses to viral infections and stress conditions. It was first identified in the 1990s as part of the interferon (IFN)-α/β signaling pathway, which is central to innate immunity. IFI6 is encoded by a gene located on human chromosome 1p35 and consists of two exons, producing a 6.9 kDa protein with a hydrophobic N-terminal region and a conserved C-terminal domain.
Functionally, IFI6 exhibits antiviral properties, particularly against RNA viruses like hepatitis C virus (HCV) and human immunodeficiency virus (HIV). It localizes to the endoplasmic reticulum (ER) and mitochondria, where it modulates ER stress responses and inhibits apoptosis by stabilizing mitochondrial membranes. This antiapoptotic activity enables infected cells to survive longer, paradoxically facilitating viral persistence in some contexts. For example, IFI6 is hijacked by HCV to promote chronic infection by delaying host cell death.
Recombinant IFI6 protein is produced using expression systems (e.g., E. coli or mammalian cells) for structural and functional studies. Its applications span virology research, drug discovery for viral diseases, and investigations into cancer biology, as IFI6 overexpression has been observed in breast and ovarian cancers. Recent studies also explore its role in COVID-19 pathogenesis and autoimmune disorders. Despite progress, the precise molecular mechanisms underlying IFI6's dual roles in antiviral defense and pathology remain an active area of research, highlighting its complexity in cellular homeostasis and disease progression.
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