| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT38 |
| Uniprot No | O76015 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-456aa |
| 氨基酸序列 | TSSYSSSSCPLGCTMAPGARNVSVSPIDIGCQPGAEANIAPMCLLANVAHANRVRVGSTPLGRPSLCLPPTCHTACPLPGTCHIPGNIGICGAYGENTLNGHEKETMQFLNDRLANYLEKVRQLEQENAELEATLLERSKCHESTVCPDYQSYFHTIEELQQKILCSKAENARLIVQIDNAKLAADDFRIKLESERSLRQLVEADKCGTQKLLDDATLAKADLEAQQESLKEEQLSLKSNHEQEVKILRSQLGEKLRIELDIEPTIDLNRVLGEMRAQYEAMLETNRQDVEQWFQAQSEGISLQDMSCSEELQCCQSEILELRCTVNALEVERQAQHTLKDCLQNSLCEAEDRFGTELAQMQSLISNVEEQLSEIRADLERQNQEYQVLLDVKTRLENEIATYRNLLESEDCKLPCNPCSTSPSCVTAPCAPRPSCGPCTTCGPTCGASTTGSRF |
| 预测分子量 | 66.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与KRT38重组蛋白相关的文献信息及摘要概括(基于公开文献的模拟示例，具体文献可能需要通过学术数据库核实)：
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1. **文献名称**: *"Cloning and expression of human keratin KRT38 in Escherichia coli for antibody production"*
**作者**: Smith J, et al.
**摘要**: 该研究报道了通过PCR扩增KRT38基因片段，构建重组质粒并在大肠杆菌中表达KRT38蛋白。纯化后的重组蛋白用于免疫动物，成功制备出特异性识别KRT38的多克隆抗体，验证了其在毛囊组织中的表达定位。
2. **文献名称**: *"Functional characterization of recombinant KRT38 in hair follicle differentiation"*
**作者**: Lee H, et al.
**摘要**: 研究利用哺乳动物细胞表达系统(如HEK293)重组表达了KRT38蛋白，发现其与KRT75等II型角蛋白相互作用，形成角蛋白纤维网络。实验表明KRT38在毛囊角质形成细胞分化过程中可能调控细胞骨架稳定性。
3. **文献名称**: *"Proteomic analysis of KRT38 mutations linked to monilethrix-like hair disorders"*
**作者**: Garcia R, et al.
**摘要**: 通过重组表达携带致病突变的KRT38蛋白，结合质谱分析发现突变体蛋白的磷酸化修饰异常，导致角蛋白纤维组装缺陷，提示KRT38基因变异可能通过破坏毛发角蛋白结构引发遗传性毛发疾病。
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**注意**：以上文献为模拟示例，实际研究中KRT38的专门报道可能较少，建议结合关键词“KRT38 recombinant protein”、“keratin 38 expression”在 **PubMed** 或 **Web of Science** 检索最新文献。若需具体文献DOI或全文链接，请提供更多研究背景。
**Background of KRT38 Recombinant Protein**
KRT38. or Keratin 38. is a member of the keratin family, a group of intermediate filament proteins critical for maintaining structural integrity and mechanical stability in epithelial cells. As a type I acidic keratin, KRT38 pairs with type II basic keratins to form heteropolymers, creating a cytoskeletal network that protects cells from stress and regulates cellular signaling. While keratins like KRT5. KRT14. and KRT18 are well-studied in skin, hair, and epithelial tissues, KRT38 has garnered attention for its potential tissue-specific roles, particularly in specialized epithelia such as the tongue or stratified mucosal surfaces.
Recombinant KRT38 is produced using expression systems like *E. coli* or mammalian cells, enabling controlled study of its biochemical properties and interactions. The protein is often purified via affinity tags (e.g., His-tag) and validated through techniques like SDS-PAGE and Western blotting. Its recombinant form bypasses challenges associated with isolating native KRT38 from tissues, which is often low in abundance and complex to extract.
Research on KRT38 focuses on its role in epithelial differentiation, barrier function, and disease. Mutations in keratins are linked to disorders like epidermolysis bullosa and ichthyosis, but KRT38’s specific pathological relevance remains under exploration. Recombinant KRT38 serves as a tool to investigate its involvement in cellular stress responses, wound healing, or cancer progression, where keratin expression profiles often shift. Additionally, it aids in developing antibodies, diagnostic assays, or therapeutic strategies targeting keratin-related pathologies. Further studies are needed to fully elucidate its biological significance and translational potential.
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