| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MT2A |
| Uniprot No | P02795 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-59aa |
| 氨基酸序列 | MDPNCSCAAGDSCTCAGSCKCKECKCTSCKKSCCSCCPVGCAKCAQGCICKGASDKCSC |
| 预测分子量 | 7.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MT2A重组蛋白的示例性参考文献(注：部分文献信息为概括性示例，实际引用请通过学术数据库核实)：
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1. **标题**: *"Recombinant human MT2A protein attenuates oxidative stress and inflammation in a cellular model of Parkinson's disease"*
**作者**: Smith J.R., et al.
**摘要**: 研究通过大肠杆菌表达系统成功重组表达了人源MT2A蛋白，并验证其体外抗氧化活性。实验表明MT2A重组蛋白可通过螯合游离金属离子和清除ROS，显著减少神经细胞凋亡，提示其在神经退行性疾病中的潜在治疗价值。
2. **标题**: *"Expression and purification of functional MT2A in Pichia pastoris: Implications for heavy metal detoxification"*
**作者**: Lee H., Kim S.
**摘要**: 利用毕赤酵母系统高效表达MT2A重组蛋白，优化纯化工艺后获得高纯度产物。功能实验证实该蛋白对镉、汞等重金属具有强结合能力，为开发重金属中毒的解毒剂提供了实验依据。
3. **标题**: *"Structural and functional characterization of MT2A recombinant protein in cancer drug resistance"*
**作者**: González A., et al.
**摘要**: 通过X射线晶体学解析MT2A重组蛋白的三维结构，并发现其过表达可降低顺铂等化疗药物对癌细胞的毒性，提示MT2A可能通过调节细胞内金属稳态参与肿瘤耐药性机制。
4. **标题**: *"MT2A recombinant protein enhances wound healing by modulating zinc homeostasis in diabetic mice"*
**作者**: Wang Y., et al.
**摘要**: 研究利用真核表达系统制备MT2A重组蛋白，动物实验表明其通过调控锌离子分布促进糖尿病模型小鼠的皮肤创面修复，为慢性伤口治疗提供了新策略。
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建议通过**PubMed**、**Web of Science**或**Google Scholar**检索关键词“MT2A recombinant protein”“metallothionein 2A expression”以获取真实文献数据。
**Background of Recombinant MT2A Protein**
Metallothionein 2A (MT2A) is a small, cysteine-rich protein belonging to the metallothionein family, which plays critical roles in metal ion homeostasis, detoxification of heavy metals, and protection against oxidative stress. MT2A binds essential (zinc, copper) and toxic (cadmium, mercury) metals through its thiol groups, regulating their cellular distribution and mitigating toxicity. It also acts as a scavenger of free radicals, contributing to cellular defense mechanisms under stress conditions.
Recombinant MT2A protein is produced using genetic engineering techniques, typically by expressing the human *MT2A* gene in bacterial (e.g., *E. coli*) or mammalian cell systems. Bacterial systems offer cost-effective production but may require refolding to achieve functional metal-binding capacity. Mammalian systems, while more complex, ensure proper post-translational modifications and structural fidelity. The purified protein is characterized for metal-binding stoichiometry, stability, and antioxidant activity.
Research on recombinant MT2A spans biomedical applications, including studies on neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), where dysregulated metal metabolism and oxidative stress are implicated. It is also explored for therapeutic potential in heavy metal poisoning and as a biomarker for metal-related disorders. Additionally, MT2A’s role in cancer progression, particularly in drug resistance and apoptosis regulation, is under investigation.
Despite its promise, challenges remain in optimizing expression yields, maintaining functional stability, and understanding tissue-specific interactions. Advances in recombinant technology and structural biology continue to enhance its applicability in both experimental and clinical settings.
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