| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYEF2 |
| Uniprot No | Q9P2K5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 372-576aa |
| 氨基酸序列 | GGMNRIGGGIGFGGLEAMNSMGGFGGVGRMGELYRGAMTSSMERDFGRGDIGINQGFGDSFGRLGSAMIGGFAGRIGSSNMGPVGSGISGGMGSMNSVTGGMGMGLDRMSSSFDRMGPGIGAILERSIDMDRGFLSGPMGSGMRERIGSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMENGKSKGCGTVRFDSPESAE |
| 预测分子量 | 37.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于MYEF2重组蛋白的参考文献示例(仅供参考，非真实文献)：
1. **文献名称**：*Recombinant MYEF2 Protein Promotes Skeletal Muscle Differentiation via RNA Binding*
**作者**：Chen L, et al.
**摘要**：本研究通过在大肠杆菌中表达并纯化MYEF2重组蛋白，发现其通过结合特定mRNA调控肌细胞分化，体外实验证实其促进成肌细胞融合及肌管形成。
2. **文献名称**：*Structural Insights into MYEF2-DNA Interaction Using Recombinant Protein Crystallography*
**作者**：Sato K, et al.
**摘要**：首次解析了重组MYEF2蛋白的晶体结构，揭示了其N端结构域与DNA靶序列的结合模式，为理解其在基因沉默中的功能提供结构基础。
3. **文献名称**：*MYEF2 Recombinant Protein Suppresses Tumor Growth in Glioblastoma Models*
**作者**：Wang Y, et al.
**摘要**：利用哺乳动物细胞系表达MYEF2重组蛋白，发现其通过抑制致癌RNA稳定性延缓胶质母细胞瘤进展，提示其作为潜在治疗靶点。
4. **文献名称**：*Development of a MYEF2 Recombinant Protein-Based Screening Assay for Neuromuscular Disorders*
**作者**：Rodriguez M, et al.
**摘要**：构建了基于MYEF2重组蛋白的高通量筛选平台，用于筛选调控肌肉特异性RNA剪接的小分子化合物，助力罕见肌病药物开发。
**注意**：以上文献为模拟生成，实际研究需通过PubMed、Web of Science等数据库检索关键词“MYEF2 recombinant”或“MYEF2 protein function”获取。
MYEF2 (Myelin Expression Factor 2) is a transcription factor primarily associated with the regulation of myelination in the central and peripheral nervous systems. It belongs to the MYEF family of DNA-binding proteins and is known to interact with the myelin basic protein (MBP) gene, a critical component of myelin sheaths that insulate nerve fibers. MYEF2 contains a conserved SCAN domain, which mediates protein-protein interactions, and a zinc finger motif enabling sequence-specific DNA binding. Its function extends to repressing or modulating the transcription of myelin-related genes, thereby influencing oligodendrocyte differentiation and myelin maintenance.
Recombinant MYEF2 protein is engineered using expression systems like E. coli or mammalian cells to produce purified, biologically active forms for research. This protein is instrumental in studying molecular mechanisms of myelination, demyelinating disorders (e.g., multiple sclerosis), and neural regeneration. Researchers utilize it to investigate MYEF2’s role in gene regulation, its interaction with chromatin modifiers, and its potential as a therapeutic target. Structural studies of the recombinant protein also aid in mapping functional domains and designing inhibitors or activators. Despite its established role in myelination, MYEF2’s broader regulatory functions in neural development and disease pathways remain an active area of exploration, highlighting its relevance in both basic and applied neuroscience.
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