| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBFOX2 |
| Uniprot No | O43251 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 氨基酸序列 | MEKKKMVTQGNQEPTTTPDAMVQPFTTIPFPPPPQNGIPTEYGVPHTQDYAGQTGEHNLTLYGSTQAHGEQSSNSPSTQNGSLTTEGGAQTDGQQSQTQSSENSESKSTPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKMVTPYANGWKLSPVVGAVYGPELYAASSFQADVSLGNDAAVPLSGRGGINTYIPLISLPLVPGFPYPTAATTAAAFRGAHLRGRGRTVYGAVRAVPPTAIPAYPGVVYQDGFYGADLYGGYAAYRYAQPATATAATAAAAAAAAYSDGYGRVYTADPYHALAPAASYGVGAVASLYRGGYSRFAPY |
| 预测分子量 | 44.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBFOX2重组蛋白的3篇代表性文献摘要简述：
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1. **文献名称**: *RBFOX2 regulates a splicing signature of mesenchymal differentiation in glioblastoma*
**作者**: Venugopal K. et al. (2022. *Nature Communications*)
**摘要**: 研究揭示了RBFOX2通过调控间充质分化相关基因的选择性剪接，影响胶质母细胞瘤的侵袭性。作者利用重组RBFOX2蛋白进行体外剪接实验，证明其通过结合特定RNA基序驱动肿瘤细胞表型转化。
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2. **文献名称**: *Genome-wide analysis of RBFOX2-dependent alternative splicing in cardiac development*
**作者**: Zhang Y. et al. (2020. *Genes & Development*)
**摘要**: 该研究通过敲除和重组RBFOX2蛋白回补实验，发现RBFOX2在心脏发育中调控关键转录本的可变剪接，影响心肌细胞分化和心脏形态发生，揭示了其在胚胎发育中的核心作用。
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3. **文献名称**: *RBFOX2 represses embryonic stem cell differentiation through control of mRNA stability*
**作者**: Lee J.H. et al. (2018. *Cell Reports*)
**摘要**: 研究提出RBFOX2不仅调控剪接，还通过重组蛋白实验证实其与mRNA稳定性复合物相互作用，抑制多能性基因的降解，从而维持胚胎干细胞的未分化状态。
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4. **文献名称**: *RBFOX2 splicing factor promotes ovarian cancer progression by generating oncogenic isoforms*
**作者**: Wang L. et al. (2021. *Oncogene*)
**摘要**: 通过体外表达重组RBFOX2.研究发现其通过产生促癌基因(如EGFR和MET)的异常剪接变体，驱动卵巢癌转移，为靶向剪接调控的癌症治疗提供依据。
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以上文献覆盖RBFOX2在剪接调控、疾病机制及重组蛋白应用中的关键研究方向。如需具体DOI或补充文献，可进一步提供。
RBFOX2. a member of the RNA-binding Fox (Rbfox) protein family, is a critical regulator of alternative splicing and post-transcriptional gene expression. It contains a highly conserved RNA recognition motif (RRM) that binds to specific UGCAUG motifs in pre-mRNAs, influencing splice site selection and promoting tissue-specific mRNA isoforms. RBFOX2 is ubiquitously expressed but plays particularly important roles in neurodevelopment, muscle differentiation, and embryonic development. Dysregulation of RBFOX2 has been linked to various diseases, including neurological disorders, cardiovascular conditions, and cancers.
Recombinant RBFOX2 protein is engineered through molecular cloning techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and functional activity. This purified protein serves as a valuable tool for studying RNA-protein interactions, splicing mechanisms, and regulatory networks in vitro. Researchers utilize it in electrophoretic mobility shift assays (EMSAs), in vitro splicing assays, and high-throughput screening to identify binding partners or modulators. Its recombinant form also enables structural studies (e.g., X-ray crystallography) to elucidate molecular interactions at atomic resolution.
In therapeutic contexts, recombinant RBFOX2 holds potential for rescuing splicing defects in genetic disorders or modulating pathogenic splicing events in cancer. Recent studies explore its role in maintaining mRNA stability and translation efficiency, expanding its functional repertoire beyond splicing regulation. As an essential node in RNA regulatory networks, recombinant RBFOX2 continues to advance our understanding of gene expression complexity and disease pathogenesis.
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