| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS27 |
| Uniprot No | P42677 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-84aa |
| 氨基酸序列 | PLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH |
| 预测分子量 | 36.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS27重组蛋白的3篇参考文献示例(注：文献为模拟虚构，仅供参考格式)：
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1. **文献名称**：*"Recombinant Human RPS27: Cloning, Expression, and Functional Analysis in DNA Repair"*
**作者**：Wang, L. et al. (2016)
**摘要**：本研究成功克隆并表达了人源RPS27重组蛋白，利用大肠杆菌系统进行纯化，并发现其通过调控MDM2-p53通路参与DNA损伤修复，为癌症治疗提供潜在靶点。
2. **文献名称**：*"RPS27 Acts as a Ubiquitin Fusion Protein in Ribosome Biogenesis"*
**作者**：Johnson, R. & Smith, T. (2018)
**摘要**：通过重组RPS27蛋白的体外实验，验证了其N端泛素样结构域在核糖体组装中的关键作用，揭示了泛素蛋白酶体系统对核糖体合成的调控机制。
3. **文献名称**：*"Overexpression of Recombinant RPS27 Promotes Tumor Growth in Colorectal Cancer Models"*
**作者**：Zhang, Y. et al. (2020)
**摘要**：构建了RPS27重组蛋白的真核表达体系，证实其在结直肠癌细胞中过表达可激活AKT/mTOR信号通路，促进肿瘤细胞增殖和迁移。
4. **文献名称**：*"Structural Insights into RPS27 via Recombinant Protein Crystallography"*
**作者**：Lee, H. et al. (2022)
**摘要**：利用重组RPS27蛋白的晶体结构解析，揭示了其与核糖体RNA结合的分子机制，为靶向核糖体功能的药物设计提供了结构基础。
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(实际研究中建议通过PubMed或Web of Science检索真实文献。)
**Background of RPS27 Recombinant Protein**
RPS27 (Ribosomal Protein S27) is a component of the 40S ribosomal subunit, playing a critical role in protein biosynthesis by facilitating mRNA decoding and ribosome assembly. Beyond its canonical ribosomal function, RPS27 has been implicated in diverse cellular processes, including DNA repair, apoptosis, and oxidative stress response. The protein contains a ubiquitin-like domain, suggesting potential roles in post-translational modifications or protein degradation pathways.
The gene encoding RPS27 is located on human chromosome 1 (1q21.3) and is evolutionarily conserved, highlighting its fundamental biological importance. Dysregulation of RPS27 expression has been associated with cancer progression, neurodegenerative disorders, and immune dysfunctions, making it a subject of interest in translational research.
Recombinant RPS27 protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to ensure proper folding and post-translational modifications. Purification techniques like affinity chromatography (e.g., His-tag systems) enable high yield and purity. This recombinant form is widely utilized in functional studies, including ribosome biogenesis assays, protein interaction analyses (e.g., with ubiquitin or tumor suppressors like p53), and drug screening for therapies targeting ribosomal dysfunction.
Furthermore, RPS27’s dual role as a ribosomal component and a multifunctional signaling molecule underscores its therapeutic potential. Research continues to explore its involvement in diseases and its utility as a biomarker or therapeutic target. The availability of recombinant RPS27 accelerates mechanistic studies, aiding in unraveling its complex biology and applications in biomedicine.
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