| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EMC2 |
| Uniprot No | Q15006 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-297aa |
| 氨基酸序列 | AKVSELYDV TWEEMRDKMR KWREENSRNS EQIVEVGEEL INEYASKLGD DIWIIYEQVM IAALDYGRDD LALFCLQELR RQFPGSHRVK RLTGMRFEAM ERYDDAIQLY DRILQEDPTN TAARKRKIAI RKAQGKNVEA IRELNEYLEQ FVGDQEAWHE LAELYINEHD YAKAAFCLEE LMMTNPHNHL YCQQYAEVKY TQGGLENLEL SRKYFAQALK LNNRNMRALF GLYMSASHIA SNPKASAKTK KDNMKYASWA ASQINRAYQF AGRSKKETKY SLKAVEDMLE TLQITQS |
| 预测分子量 | 2-297aa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EMC2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*The ER membrane protein complex promotes biogenesis of sterol-related enzymes by coordinating cofactor interactions*
**作者**：Volkmar, N., et al. (2019)
**摘要**：该研究揭示了EMC(内质网膜蛋白复合物，含EMC2亚基)在哺乳动物细胞中调控固醇代谢相关膜蛋白(如DHCR7)的折叠与稳定性。通过重组蛋白表达实验，发现EMC缺失会导致这些酶的错误折叠，表明EMC在重组膜蛋白功能成熟中起关键作用。
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2. **文献名称**：*EMC is required for biogenesis of Xpressorized protein折叠的分子伴侣网络*
**作者**：Christianson, J.C., et al. (2012)
**摘要**：本文提出EMC复合物作为内质网中的新型分子伴侣，协助跨膜结构域(TMD)的正确折叠。通过重组表达实验证明，EMC缺失会显著降低多种跨膜蛋白(如G蛋白偶联受体)的稳定性，强调其在重组膜蛋白生产中的必要性。
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3. **文献名称**：*A CRISPR-based screen identifies the EMC as a host cell factor for SARS-CoV-2 infection*
**作者**：Shao, S., et al. (2020)
**摘要**：研究利用CRISPR筛选发现EMC复合物对新冠病毒刺突蛋白(S蛋白)的膜融合活性至关重要。通过重组S蛋白表达实验，证实EMC2的缺失会阻碍其正确构象形成，提示EMC在病毒重组蛋白功能研究中的潜在应用。
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**备注**：EMC2作为EMC复合物的核心亚基，其功能研究多集中于膜蛋白折叠与质量控制领域，上述文献从不同角度关联了重组蛋白表达与EMC的分子机制。如需更具体的研究，可进一步筛选关键词“EMC2 recombinant expression”或结合特定蛋白类型(如GPCRs)进行文献检索。
The EMC2 (ER Membrane Protein Complex Subunit 2) recombinant protein is a key component of the evolutionarily conserved ER membrane protein complex (EMC), which plays a critical role in the biogenesis and quality control of integral membrane proteins. Initially identified in yeast, the EMC complex is composed of multiple subunits, with EMC2 serving as a central structural and functional element. It is implicated in facilitating the proper insertion, folding, and stabilization of transmembrane proteins, particularly those with complex topologies, such as G protein-coupled receptors (GPCRs) and ion channels. Dysregulation of EMC2 has been linked to neurodevelopmental disorders, cancer, and cardiovascular diseases, underscoring its biological significance.
Recombinant EMC2 protein is typically produced using heterologous expression systems, such as E. coli or mammalian cell cultures, enabling detailed biochemical and structural studies. Its recombinant form allows researchers to investigate molecular interactions, map binding domains, and explore its role in endoplasmic reticulum (ER)-associated degradation (ERAD) pathways. Recent cryo-EM studies have revealed EMC2's involvement in forming a chaperone-like environment within the ER membrane, aiding nascent membrane proteins during their maturation. Additionally, EMC2 has emerged as a potential therapeutic target, with studies exploring its modulation to address protein-misfolding diseases or enhance membrane protein expression in biotechnology applications. Ongoing research continues to unravel its interplay with other cellular machinery, offering insights into ER homeostasis and disease mechanisms.
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