| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF165 |
| Uniprot No | P49910 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-485aa |
| 氨基酸序列 | MATEPKKAAAQNSPEDEGLLIVKIEEEEFIHGQDTCLQRSELLKQELCRQLFRQFCYQDSPGPREALSRLRELCCQWLKPEIHTKEQILELLVLEQFLTILPGDLQAWVHEHYPESGEEAVTILEDLERGTDEAVLQVQAHEHGQEIFQKKVSPPGPALNVKLQPVETKAHFDSSEPQLLWDCDNESENSRSMPKLEIFEKIESQRIISGRISGYISEASGESQDICKSAGRVKRQWEKESGESQRLSSAQDEGFGKILTHKNTVRGEIISHDGCERRLNLNSNEFTHQKSCKHGTCDQSFKWNSDFINHQIIYAGEKNHQYGKSFKSPKLAKHAAVFSGDKTHQCNECGKAFRHSSKLARHQRIHTGERCYECNECGKSFAESSDLTRHRRIHTGERPFGCKECGRAFNLNSHLIRHQRIHTREKPYECSECGKTFRVSSHLIRHFRIHTGEKPYECSECGRAFSQSSNLSQHQRIHMRENLLM |
| 预测分子量 | 71.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于ZNF165重组蛋白的参考文献示例(实际文献需通过学术数据库查询确认)：
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1. **文献名称**: *ZNF165重组蛋白在乳腺癌细胞中的功能表征*
**作者**: Smith J, et al. (2015)
**摘要**: 本研究通过原核系统表达ZNF165重组蛋白，发现其通过调控TGF-β信号通路抑制乳腺癌细胞侵袭，提示其潜在肿瘤抑制功能。
2. **文献名称**: *ZNF165重组蛋白的晶体结构及DNA结合特性分析*
**作者**: Chen L, et al. (2018)
**摘要**: 解析了ZNF165重组蛋白的锌指结构域晶体结构，证实其特异性结合GC-rich DNA序列，为研究其在基因转录调控中的作用提供结构基础。
3. **文献名称**: *ZNF165重组蛋白在前列腺癌中的异常表达及临床意义*
**作者**: Johnson R, et al. (2020)
**摘要**: 利用重组ZNF165蛋白制备抗体，发现其在转移性前列腺癌组织中表达显著下调，且与患者预后不良相关。
4. **文献名称**: *重组ZNF165蛋白体外调控干细胞多能性的机制研究*
**作者**: Lee S, et al. (2019)
**摘要**: 通过体外实验证明，ZNF165重组蛋白通过抑制Oct4启动子活性影响胚胎干细胞的自我更新能力。
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注：以上内容为学术写作示例，实际文献需通过PubMed、Web of Science等平台以“ZNF165 recombinant protein”等关键词检索核实。
ZNF165 recombinant protein is derived from the ZNF165 gene, which encodes a member of the zinc finger protein family, a group of transcription factors characterized by conserved zinc-binding domains critical for DNA or RNA interactions. ZNF165 contains multiple C2H2-type zinc finger motifs, which are typically involved in sequence-specific nucleic acid binding, and a SCAN domain, a structural feature implicated in protein-protein interactions and regulatory functions. The gene is located on human chromosome 6 (6p21.1) and is expressed in various tissues, though its biological roles remain incompletely understood.
Studies suggest ZNF165 may play context-dependent roles in cellular processes, including transcriptional regulation, chromatin remodeling, and possibly tumorigenesis. For example, it has been linked to cancer progression in certain contexts, with elevated expression observed in breast and prostate cancers, though its precise mechanism remains unclear. Some reports indicate ZNF165 may promote epithelial-mesenchymal transition (EMT) and metastasis, while others propose tumor-suppressive effects, highlighting functional complexity. Recombinant ZNF165 protein, often produced in systems like E. coli or mammalian cells, enables in vitro studies to dissect its molecular interactions, DNA-binding specificity, and regulatory networks. Researchers utilize it to investigate post-translational modifications, subcellular localization, and signaling pathways influenced by ZNF165. Additionally, it serves as an antigen for antibody development and a tool for screening small molecules targeting zinc finger proteins. Despite progress, challenges persist in clarifying its physiological targets, tissue-specific functions, and therapeutic relevance, necessitating further structural and functional analyses.
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