| 纯度 | >90%SDS-PAGE. |
| 种属 | E. coli |
| 靶点 | ctfA |
| Uniprot No | P33752 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-218aa |
| 氨基酸序列 | MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNIKNLTIISNDTCYPNTGIGKLISNNQVKKLIASYIGSNPDTGKKLFNNELEVELSPQGTLVERIRAGGSGLGGVLTKTGLGTLIEKGKKKISINGTEYLLELPLTADVALIKGSIVDEAGNTFYKGTTKNFNPYMAMAAKTVIVEAENLVSCEKLEKEKAMTPGVLINYIVKEPA |
| 预测分子量 | 23,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ctfA重组蛋白的3篇文献示例(内容为虚构模拟，仅供参考)：
1. **《Functional characterization of recombinant ctfA from Clostridium acetobutylicum》**
- 作者：Smith J, et al.
- 摘要：研究通过大肠杆菌系统重组表达了ctfA基因编码的辅酶A转移酶，证实其催化丁酸和乙酸转化为丁酰-CoA和乙酰-CoA的能力，为优化丙酮丁醇发酵工艺提供依据。
2. **《Structural insights into the ctfA protein in solventogenic clostridia》**
- 作者：Wang L, et al.
- 摘要：利用X射线晶体学解析了ctfA重组蛋白的三维结构，揭示了其底物结合域的关键氨基酸残基，阐明了该酶在溶剂生成代谢途径中的分子机制。
3. **《Heterologous expression and purification of ctfA for biofuel production》**
- 作者：Zhang Y, et al.
- 摘要：优化了ctfA重组蛋白在毕赤酵母中的表达条件，纯化后酶活提高3倍，证明其在体外合成丁醇前体的高效性，为生物燃料开发提供新策略。
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**注**：以上文献为示例，实际研究中需通过数据库(如PubMed、Web of Science)以“ctfA recombinant protein”或“ctfA CoA-transferase”等关键词检索真实文献。若需具体论文，可补充说明研究方向(如微生物代谢、酶工程等)。
**Background of ctfA Recombinant Protein**
The *ctfA* gene encodes a key enzyme, Coenzyme A-transferase (CtFA), primarily studied in solventogenic clostridia such as *Clostridium acetobutylicum*. This enzyme plays a critical role in the acetone-butanol-ethanol (ABE) fermentation pathway, a metabolic process converting sugars into solvents. CtFA catalyzes the transfer of coenzyme A (CoA) from acetoacetyl-CoA to acetate or butyrate, forming acetyl-CoA or butyryl-CoA, which are essential intermediates for acetone and butanol production.
Recombinant CtFA protein is engineered to enable efficient expression in heterologous hosts like *Escherichia coli*. This approach circumvents challenges associated with low native expression levels in clostridia and simplifies protein purification. The recombinant protein is typically produced using plasmid vectors with inducible promoters (e.g., T7 or lac), fused to affinity tags (e.g., His-tag) for streamlined purification via immobilized metal affinity chromatography (IMAC).
Studies on recombinant CtFA focus on elucidating its structural and functional properties, substrate specificity, and regulatory mechanisms. Its application extends to metabolic engineering for enhancing solvent yields in biofuel production. Additionally, CtFA serves as a model enzyme for understanding CoA-transferase family dynamics, contributing to synthetic biology and industrial biotechnology.
The development of recombinant CtFA has facilitated biochemical assays, crystallography, and mutagenesis studies, providing insights into its catalytic mechanism. Its robustness and adaptability make it valuable for both fundamental research and biotechnological innovations in sustainable energy and chemical synthesis.
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