| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DREB2C |
| Uniprot No | Q8LFR2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-341aa |
| 氨基酸序列 | MPSEIVDRKRKSRGTRDVAEILRQWREYNEQIEAESCIDGGGPKSIRKPPPKGSRKGCMKGKGGPENGICDYRGVRQRRWGKWVAEIREPDGGARLWLGTFSSSYEAALAYDEAAKAIYGQSARLNLPEITNRSSSTAATATVSGSVTAFSDESEVCAREDTNASSGFGQVKLEDCSDEYVLLDSSQCIKEELKGKEEVREEHNLAVGFGIGQDSKRETLDAWLMGNGNEQEPLEFGVDETFDINELLGILNDNNVSGQETMQYQVDRHPNFSYQTQFPNSNLLGSLNPMEIAQPGVDYGCPYVQPSDMENYGIDLDHRRFNDLDIQDLDFGGDKDVHGST |
| 预测分子量 | 43.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **DREB2C重组蛋白** 的3篇代表性文献摘要信息(模拟内容，供参考)：
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1. **文献名称**：*Functional characterization of DREB2C as a transcription factor involved in drought stress response in Arabidopsis*
**作者**：Yamaguchi-Shinozaki, K., et al.
**摘要**：研究通过重组DREB2C蛋白的体外结合实验，验证其特异性结合DRE/CRT顺式作用元件的能力，并发现其过表达显著增强拟南芥对干旱和高盐胁迫的耐受性，同时激活胁迫响应基因(如RD29A)的表达。
2. **文献名称**：*Heterologous expression and purification of DREB2C recombinant protein from E. coli for structural analysis*
**作者**：Liu, T., & Chen, M.
**摘要**：报道了在大肠杆菌系统中高效表达重组DREB2C蛋白的优化策略，通过His标签纯化获得高纯度蛋白，并利用圆二色谱(CD)分析其二级结构，证实其具有典型的AP2/ERF结构域特征。
3. **文献名称**：*DREB2C interacts with SnRK2 kinases to regulate ABA signaling in rice*
**作者**：Park, S., et al.
**摘要**：通过酵母双杂交和重组蛋白互作实验，发现水稻DREB2C与SnRK2激酶在ABA信号通路中直接互作，协同调控下游基因表达，揭示了其在非生物胁迫响应中的新调控机制。
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如需具体文献，建议通过 **PubMed/Google Scholar** 检索关键词 *DREB2C recombinant protein* 或结合研究领域筛选。
The DREB2C (Dehydration-Responsive Element-Binding Protein 2C) recombinant protein is a key focus in plant stress biology due to its regulatory role in abiotic stress responses. DREB proteins belong to the AP2/ERF transcription factor family, which binds to dehydration-responsive elements (DREs) in the promoters of stress-inducible genes. Among the DREB subfamily, DREB2C is particularly notable for its involvement in mediating plant adaptation to environmental stresses such as drought, high salinity, and extreme temperatures. Unlike DREB1-type proteins, which are primarily induced by cold, DREB2 members like DREB2C are activated under osmotic stress and heat, making them critical targets for improving crop resilience in changing climates.
Recombinant DREB2C is engineered through genetic cloning and heterologous expression systems (e.g., E. coli or yeast) to study its structure, DNA-binding properties, and interaction networks. Structurally, it contains a conserved AP2/ERF DNA-binding domain and a nuclear localization signal. Studies reveal that DREB2C regulates downstream genes like RD29A and COR15A, which encode protective proteins to stabilize cellular structures under stress. However, its activity is tightly controlled post-translationally; for instance, under non-stress conditions, negative regulatory domains limit its activation until stress signals trigger modifications.
Research on recombinant DREB2C aims to unravel its activation mechanisms, including phosphorylation and protein degradation pathways. Its overexpression in transgenic plants has shown enhanced stress tolerance, though excessive activation may impair growth, highlighting the need for balanced engineering. Biotechnological applications leverage DREB2C to develop stress-resistant crops, addressing food security challenges. Current studies also explore synergies with other transcription factors and epigenetic regulators to optimize stress-response networks. Despite progress, challenges remain in understanding tissue-specific roles and avoiding pleiotropic effects during agricultural deployment.
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