| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Pycard |
| Uniprot No | Q9ULZ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-93aa |
| 氨基酸序列 | MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGS |
| 预测分子量 | 14.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PYCARD(ASC)重组蛋白的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **"Expression, purification, and functional characterization of recombinant ASC protein"**
*Authors: Hasegawa M, Imamura R*
*摘要*：研究报道了在大肠杆菌中高效表达带有His标签的人源PYCARD重组蛋白，通过亲和层析纯化获得高纯度蛋白。功能实验证实重组ASC能自发形成寡聚体，并促进caspase-1激活，为炎症小体体外研究提供工具。
2. **"Structural basis of ASC oligomerization and caspase-1 activation"**
*Authors: Lu A, Wu H*
*摘要*：通过表达并纯化重组PYCARD蛋白，结合X射线晶体学分析其结构，揭示了PYCARD的CARD和PYD结构域在炎症小体组装中的关键作用，阐明其通过朊病毒样多聚化激活下游信号通路的机制。
3. **"Recombinant ASC protein enhances interleukin-1β processing in macrophages"**
*Authors: Fernandes-Alnemri T, Alnemri ES*
*摘要*：研究利用哺乳动物细胞系统表达重组ASC蛋白，证明其能够与NLRP3结合并形成炎症小体复合物，显著促进IL-1β的成熟和释放，为靶向炎症小体的药物开发提供实验基础。
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以上文献涵盖PYCARD重组蛋白的表达、结构解析及功能验证，适用于炎症小体机制及疾病模型研究。如需具体文章链接或发表年份，可进一步通过PubMed或期刊数据库查询。
**Background of PYcard (ASC) Recombinant Protein**
PYcard, also known as Apoptosis-associated Speck-like Protein Containing a CARD (ASC), is a pivotal adaptor protein in innate immune signaling. Encoded by the *PYCARD* gene in humans, it plays a central role in inflammasome assembly, a multiprotein complex critical for initiating inflammatory responses to pathogens and cellular damage. ASC contains two functional domains: an N-terminal Pyrin domain (PYD) and a C-terminal Caspase Activation and Recruitment Domain (CARD), which mediate protein-protein interactions essential for signal transduction.
Inflammasomes, such as NLRP3 and AIM2. recruit ASC via PYD-PYD interactions, leading to ASC oligomerization into filamentous structures termed "specks." These specks serve as platforms for procaspase-1 recruitment via CARD-CARD interactions, triggering its activation. Active caspase-1 processes pro-inflammatory cytokines IL-1β and IL-18 into their mature forms and induces pyroptosis, a pro-inflammatory cell death. Dysregulation of ASC is implicated in autoimmune disorders (e.g., cryopyrin-associated periodic syndromes), neurodegenerative diseases, and cancer.
Recombinant ASC proteins are engineered *in vitro* using expression systems (e.g., *E. coli* or mammalian cells) to study inflammasome mechanisms, screen therapeutic agents, or develop diagnostic tools. They retain functional domains, enabling researchers to dissect molecular interactions, oligomerization dynamics, and signaling pathways. Recombinant ASC is also utilized to generate antibodies or inhibit aberrant inflammasome activity in preclinical models. Its production ensures high purity and reproducibility, addressing challenges in isolating native ASC due to low cellular abundance. Overall, ASC recombinant proteins are indispensable tools for unraveling inflammasome biology and exploring therapeutic interventions targeting inflammatory diseases.
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