| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PITPNM3 |
| Uniprot No | Q9BZ71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 387-597aa |
| 氨基酸序列 | LGRFDFDVSDFFLFGSPLGLVLAMRRTVLPGLDGFQVRPACSQVYSFFHCADPSASRLEPLLEPKFHLVPPVSVPRYQRFPLGDGQSLLLADALHTHSPLFLEGSSRDSPPLLDAPASPPQASRFQRPGRRMSEGSSHSESSESSDSMAPVGASRITAKWWGSKRIDYALYCPDVLTAFPTVALPHLFHASYWESTDVVAFILRQVMRYES |
| 预测分子量 | 30.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PITPNM3重组蛋白的3篇模拟参考文献及其摘要概括：
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1. **标题**：*Structural characterization of recombinant human PITPNM3 reveals insights into lipid-binding domains*
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组人源PITPNM3蛋白，利用X射线晶体学解析其三维结构，揭示了其磷脂酰肌醇结合域的关键氨基酸残基，为理解其脂质转运机制提供了结构基础。
2. **标题**：*PITPNM3 regulates cancer cell migration via modulation of PI3K/Akt signaling pathway*
**作者**：Chen L, et al.
**摘要**：通过体外实验发现，重组PITPNM3蛋白的过表达显著增强乳腺癌细胞的迁移能力，机制研究表明其通过激活PI3K/Akt信号通路促进肿瘤转移，提示其作为潜在治疗靶点的可能性。
3. **标题**：*Interaction of PITPNM3 with Rho GTPases in neuronal polarization*
**作者**：Zhang Y, et al.
**摘要**：该研究利用重组PITPNM3蛋白进行免疫共沉淀实验，证实其与RhoA和Cdc42等GTP酶的直接相互作用，调控神经元极化过程，为阐明其在神经发育中的作用提供了实验证据。
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以上模拟文献涵盖了结构解析、癌症机制及神经功能等方向，反映了PITPNM3重组蛋白的多领域研究价值。
**Background of PITPNM3 Recombinant Protein**
PITPNM3 (phosphatidylinositol transfer protein membrane-associated protein 3), also known as Nir3. is a member of the phosphatidylinositol transfer protein (PITP) family. These proteins play critical roles in lipid metabolism and signaling by facilitating the transfer of phosphatidylinositol (PI) or other phospholipids between membrane compartments. PITPNM3 is uniquely characterized by its N-terminal PITP-like domain and a C-terminal transmembrane region, enabling its association with cellular membranes, particularly the Golgi apparatus and endoplasmic reticulum. It is implicated in regulating phosphoinositide homeostasis, vesicular trafficking, and cellular signaling pathways, including those linked to growth factor receptors and cytoskeletal dynamics.
Recombinant PITPNM3 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research applications. Its recombinant form allows precise study of its lipid-transfer activity, interactions with signaling molecules (e.g., ARF GTPases), and role in diseases. Dysregulation of PITPNM3 has been associated with cancer progression, neurodevelopmental disorders, and retinal degeneration, making it a potential therapeutic target.
Studies leveraging recombinant PITPNM3 have clarified its structural motifs and mechanistic contributions to processes like cytokinesis, receptor trafficking, and autophagy. Its ability to rescue PI transfer defects in cellular models underscores its functional relevance. However, challenges remain in fully elucidating its tissue-specific roles and post-translational modifications. Overall, recombinant PITPNM3 serves as a vital tool for dissecting phosphoinositide-mediated cellular regulation and exploring therapeutic strategies targeting lipid signaling networks.
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