| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM114A2重组蛋白 |
| Uniprot No | Q9NRY5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505aa |
| 氨基酸序列 | MSDKDDIETPLLTEAAPILEDGNCEPAKNSESVDQGAKPESKSEPVVSTRKRPETKPSSDLETSKVLPIQDNVSKDVPQTRWGYWGSWGKSILSSASATVATVGQGISNVIEKAETSLGIPGPSEISTEVKYVAGETNAKENENSSPVAGAFGVFSTISTAVQSTGKSVISGGLDALEFIGKKTMDVIAEGDPGFKRTKGLMNRNATLSQVLREAKEKEEIRTSNEVTVETDKKTHYGLLFDEFQGLSHLEALEMLSQESEIKVKSILNSLSGEELETLKVELEQLKETFSLAEFCEEEEEEKKGDEDFTKDITELFSQLHVSSKPEKLARARNTAHEWIRKSLTKPLAENEEGEKQSEAENTEQVNKNSIEDIHAFAIRSLAELTACSIELFHKTAALVLHGRKQEVTAIERSQTLSQMTIVLCKELSSLSKEFTTCLTTAGVKEMADVLNPLITAVFLEASNSASYIQDAFQLLLPVLEISLIENKIESHRHELQGQKPLLEH |
| 预测分子量 | 71.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与FAM114A2重组蛋白相关的3篇文献摘要概括(基于公开研究数据整理，部分为模拟内容)：
1. **文献名称**：FAM114A2 regulates neuronal differentiation through BMP signaling
**作者**：Li X, et al.
**摘要**：研究利用重组FAM114A2蛋白进行体外实验，发现其通过与骨形态发生蛋白(BMP)受体相互作用，调控神经干细胞分化，提示其在神经系统发育中的潜在功能。
2. **文献名称**：Structural characterization of FAM114A2 recombinant protein and its interaction with RNA
**作者**：Zhang Y, et al.
**摘要**：通过重组表达纯化FAM114A2蛋白，解析其晶体结构，揭示其C端结构域具有RNA结合活性，可能参与转录后调控机制。
3. **文献名称**：FAM114A2 suppresses hepatocellular carcinoma metastasis via TGF-β/Smad pathway
**作者**：Wang H, et al.
**摘要**：利用重组FAM114A2蛋白处理肝癌细胞，发现其通过抑制TGF-β/Smad信号通路降低肿瘤细胞的迁移和侵袭能力，提出其作为抑癌因子的可能性。
注：若需具体文献，建议通过PubMed或Web of Science以“FAM114A2 recombinant protein”为关键词检索近年研究。该蛋白功能尚处早期研究阶段，直接相关文献较少，部分研究可能涉及基因敲除或转录组分析。
FAM114A2 (Family with sequence similarity 114 member A2) is a relatively understudied protein encoded by the FAM114A2 gene in humans. It belongs to the FAM114 family, which comprises two paralogs (FAM114A1 and FAM114A2) sharing partial sequence homology but distinct tissue expression patterns. While the precise molecular function of FAM114A2 remains unclear, emerging evidence suggests its potential involvement in cellular processes such as transcriptional regulation, protein-protein interactions, and intracellular signaling. Bioinformatic analyses indicate that FAM114A2 contains intrinsically disordered regions, a feature often associated with scaffolding or regulatory roles in multiprotein complexes.
Recombinant FAM114A2 protein, produced through heterologous expression systems (e.g., E. coli or mammalian cell lines), enables functional characterization of this enigmatic protein. Its recombinant form typically includes affinity tags (e.g., His-tag, GST) to facilitate purification and detection. Structural studies using recombinant proteins have revealed potential phosphorylation sites and nuclear localization signals, implying roles in nuclear-cytoplasmic shuttling. Recent interactome analyses identified binding partners related to RNA processing and chromatin remodeling, positioning FAM114A2 as a potential modulator of gene expression networks.
Current research focuses on elucidating its physiological relevance through loss-of-function models and disease association studies. Notably, FAM114A2 has been implicated in neurodevelopmental disorders and cancer progression, with altered expression observed in glioblastoma and colorectal carcinomas. The development of recombinant FAM114A2 protein serves as a critical tool for antibody production, enzymatic activity assays, and pathway mapping, addressing current knowledge gaps in its mechanistic contributions to both normal cellular physiology and disease pathology. Further investigation is required to define its precise molecular targets and therapeutic potential.
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