| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS16 |
| Uniprot No | Q9Y3D3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-137aa |
| 氨基酸序列 | VAAHNKCPRDGRFVEQLGSYDPLPNSHGEKLVALNLDRIRHWIGCGAHLSKPMEKLLGLAGFFPLHPMMITNAERLRRKRAREVLLASQKTDAEATDTEATET |
| 预测分子量 | 38.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPS16重组蛋白的示例参考文献(注：以下为假设性示例，实际文献需通过学术数据库查询)：
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1. **标题**: *"Expression and Functional Characterization of Recombinant MRPS16 in Mitochondrial Translation"*
**作者**: Zhang L, et al.
**摘要**: 研究通过大肠杆菌系统成功表达并纯化重组MRPS16蛋白，验证其在线粒体核糖体组装及蛋白质翻译中的关键作用，揭示其缺失导致氧化磷酸化功能受损。
2. **标题**: *"Structural Insights into MRPS16 Role in Mitochondrial Ribosome Assembly via Recombinant Protein Analysis"*
**作者**: Müller R, et al.
**摘要**: 利用重组MRPS16蛋白进行冷冻电镜结构解析，阐明其与线粒体rRNA的相互作用位点，为线粒体疾病相关突变提供分子机制解释。
3. **标题**: *"MRPS16 Mutations and Rescue by Recombinant Protein in Cardiomyopathy Models"*
**作者**: Tanaka K, et al.
**摘要**: 在心肌病细胞模型中，发现MRPS16突变导致线粒体功能障碍，通过导入重组MRPS16蛋白部分恢复ATP合成能力，提示潜在治疗策略。
4. **标题**: *"High-Yield Production of Recombinant MRPS16 for Drug Screening Applications"*
**作者**: Chen H, et al.
**摘要**: 开发哺乳动物细胞表达系统高效生产重组MRPS16.用于筛选靶向线粒体翻译的小分子化合物，推动罕见病药物开发。
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建议通过 **PubMed、Web of Science 或 Google Scholar** 检索真实文献，使用关键词 "MRPS16 recombinant protein" 或 "mitochondrial ribosomal protein S16 expression" 获取最新研究。
**Background of MRPS16 Recombinant Protein**
Mitochondrial ribosomal protein S16 (MRPS16) is a key component of the small subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis. As part of the mitochondrial ribosome, MRPS16 plays a critical role in assembling the oxidative phosphorylation (OXPHOS) system by translating mitochondrial DNA (mtDNA)-encoded subunits of electron transport chain complexes. Mitochondria, known as cellular powerhouses, rely on OXPHOS to generate ATP, and defects in mitochondrial translation machinery are linked to metabolic disorders, neurodegenerative diseases, and cancer.
The MRPS16 gene is located on chromosome 10 in humans and encodes a highly conserved protein across eukaryotes. Studies suggest that MRPS16 mutations or dysregulation may impair mitochondrial ribosome assembly, leading to reduced OXPHOS activity, compromised cellular energy production, and increased oxidative stress. Such disruptions are implicated in rare mitochondrial disorders and broader pathologies, including developmental defects and age-related conditions.
Recombinant MRPS16 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cell cultures*) to study its structure, function, and interactions. This engineered protein enables researchers to investigate MRPS16's role in mitochondrial translation, its binding partners, and its contribution to disease mechanisms. Additionally, recombinant MRPS16 serves as a tool for screening therapeutic compounds targeting mitochondrial dysfunction or for developing biomarkers in mitochondrial diseases.
Overall, MRPS16 recombinant protein is vital for advancing our understanding of mitochondrial biology and its clinical implications, bridging molecular insights with potential therapeutic strategies.
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