| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL18 |
| Uniprot No | Q07020 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-187aa |
| 氨基酸序列 | GVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGRENKTAVVVGTITDDVRVQEVPKLKVCALRVTSRARSRILRAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPHSHTKPYVRSKGRKFERARGRRASRGYK |
| 预测分子量 | 48.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL18重组蛋白的模拟参考文献示例(注：文献为虚构，仅供示例参考)：
---
1. **文献名称**：*Structural Insights into Recombinant Human RPL18 and Its Interaction with rRNA*
**作者**：Zhang Y. et al.
**摘要**：通过X射线晶体学解析了重组人源RPL18蛋白的三维结构，揭示了其保守的RNA结合域，并证明其与28S rRNA的特异性结合对核糖体大亚基组装至关重要。
---
2. **文献名称**：*Functional Role of RPL18 in Ribosome Biogenesis Revealed by CRISPR Knockout*
**作者**：Lee S. et al.
**摘要**：利用CRISPR技术敲除RPL18基因，发现其缺失导致核糖体组装缺陷和细胞增殖停滞，重组RPL18蛋白可部分恢复功能，表明其在核糖体成熟中的关键作用。
---
3. **文献名称**：*Overexpression of Recombinant RPL18 Promotes Tumor Growth in Murine Models*
**作者**：Wang X. et al.
**摘要**：在结直肠癌模型中，重组RPL18蛋白的过表达通过激活mTOR通路加速肿瘤生长，提示其作为潜在癌症治疗靶点的可能性。
---
4. **文献名称**：*Development of a High-Throughput Assay Using Recombinant RPL18 for Antiviral Drug Screening*
**作者**：Kim J. et al.
**摘要**：构建了基于重组RPL18蛋白的高通量筛选平台，筛选出可抑制病毒劫持宿主核糖体的小分子化合物，为抗病毒药物研发提供新策略。
---
如需真实文献，建议通过PubMed或Google Scholar检索关键词“recombinant RPL18”或“ribosomal protein L18”。
**Background of Recombinant RPL18 Protein**
Ribosomal protein L18 (RPL18) is a core component of the 60S large ribosomal subunit, playing a critical role in protein synthesis by facilitating ribosome assembly and stabilizing rRNA interactions. As part of the conserved ribosomal protein family, RPL18 contributes to the structural integrity of the ribosome and ensures accurate translation of mRNA into functional polypeptides. Beyond its canonical role, emerging studies suggest RPL18 may participate in extra-ribosomal functions, including cell cycle regulation, apoptosis, and viral infection responses, highlighting its potential involvement in disease pathways such as cancer.
Recombinant RPL18 protein is engineered using molecular cloning techniques, where the RPL18 gene is inserted into expression vectors (e.g., bacterial or mammalian systems) to enable large-scale production. This approach allows for the generation of highly purified, tag-fused (e.g., His-tag) or tag-free proteins, tailored for experimental needs. Recombinant RPL18 retains biological activity, making it invaluable for structural studies (e.g., X-ray crystallography), ribosome biogenesis research, and investigations into its non-cellular roles.
In biomedical research, recombinant RPL18 serves as a tool to explore mechanisms underlying diseases linked to ribosomal dysfunction, such as ribosomopathies or cancers with dysregulated translation. It is also utilized in developing targeted therapies, including antibody production or drug screening platforms. The availability of recombinant RPL18 accelerates functional studies, bridging gaps between ribosomal biology and therapeutic innovation.
×
