| 纯度 | >95%SDS-PAGE. |
| 种属 | Drosophila melanogaster |
| 靶点 | GAGA |
| Uniprot No | Q08605 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-130aa |
| 氨基酸序列 | MSLPMNSLYS LTWGDYGTSL VSAIQLLRCH GDLVDCTLAA GGRSFPAHKI VLCAASPFLL DLLKNTPCKH PVVMLAGVNA NDLEALLEFV YRGEVSVDHA QLPSLLQAAQ CLNIQGLAPQ TVTKDDYTTH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAGA重组蛋白的3篇参考文献示例(文献名称、作者及摘要概括)：
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1. **文献名称**：*The GAGA factor of Drosophila is a multifunctional protein with DNA-binding and chromatin remodeling activities*
**作者**：Benyajati, C., et al.
**摘要**：该研究通过重组表达果蝇GAGA蛋白，揭示其通过结合DNA特定序列并招募染色质重塑复合体(如NURF)，调控基因转录起始和染色质结构的功能机制。
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2. **文献名称**：*Recombinant GAGA factor mediates nucleosome displacement and enhances transcription in vitro*
**作者**：Tsukiyama, T., et al.
**摘要**：利用重组GAGA蛋白进行体外实验，证明其能够通过破坏核小体结构促进转录因子结合，并依赖ATP酶活性实现染色质动态调控。
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3. **文献名称**：*Structural insights into the DNA-binding specificity of the GAGA transcription factor*
**作者**：Omichinski, J.G., et al.
**摘要**：通过X射线晶体学解析重组GAGA蛋白的DNA结合域结构，阐明其锌指模体与靶DNA序列(CTCT位点)的特异性互作模式。
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(注：以上文献信息为示例性概括，实际引用需核对具体文献来源及细节。)
**Background of GAGA Recombinant Protein**
The GAGA protein, initially identified in *Drosophila melanogaster*, is a transcription factor critical for gene regulation during development. It binds to specific DNA sequences rich in GA repeats (hence the name "GAGA") and plays a dual role in chromatin remodeling and transcriptional activation. Discovered in the 1990s, GAGA is associated with the regulation of homeotic genes, heat shock proteins, and other developmentally important loci. Its ability to modulate chromatin structure by recruiting histone-modifying enzymes or ATP-dependent chromatin remodelers, such as the NURF complex, positions it as a key player in maintaining transcriptional competence and epigenetic memory.
Structurally, GAGA contains a BTB/POZ domain for protein interactions and a C-terminal zinc finger domain for DNA binding. Recombinant GAGA protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, DNA-binding kinetics, and interactions with cofactors. Its recombinant form enables in vitro analyses, including electrophoretic mobility shift assays (EMSAs) and chromatin reconstitution experiments, to dissect mechanisms of gene silencing/activation.
Beyond *Drosophila*, GAGA homologs exist in vertebrates, such as the human Kaiso/ZBTB family, highlighting its evolutionary conservation. Research on recombinant GAGA has implications for understanding developmental disorders, cancer (via chromatin dysregulation), and synthetic biology applications. Its role as a "pioneer factor" — opening condensed chromatin to allow access for other regulators — underscores its importance in dynamic gene expression networks. Today, GAGA recombinant protein remains a vital tool for probing chromatin dynamics and transcriptional regulation in metazoans.
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