| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IHH |
| Uniprot No | Q14623 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 203-411aa |
| 氨基酸序列 | CFPAGAQVRLESGARVALSAVRPGDRVLAMGEDGSPTFSDVLIFLDREPHRLRAFQVIETQDPPRRLALTPAHLLFTADNHTEPAARFRATFASHVQPGQYVLVAGVPGLQPARVAAVSTHVALGAYAPLTKHGTLVVEDVVASCFAAVADHHLAQLAFWPLRLFHSLAWGSWTPGEGVHWYPQLLYRLGRLLLEEGSFHPLGMSGAGS |
| 预测分子量 | 26.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IHH重组蛋白的3篇代表性文献概览：
1. **文献名称**：Production of Recombinant Indian Hedgehog (IHH) Protein and Its Role in Osteoblast Differentiation
**作者**：Y. Maeda et al.
**摘要**：研究通过哺乳动物细胞系统表达重组IHH蛋白，证实其可激活Hedgehog信号通路，显著促进间充质干细胞的成骨分化，为骨再生研究提供工具。
2. **文献名称**：A Novel Purification Strategy for Active IHH Recombinant Protein Using Insect Cell Expression
**作者**：J. Smith & R. Patel
**摘要**：开发基于杆状病毒-昆虫细胞表达系统的IHH重组蛋白生产方法，通过亲和层析获得高纯度蛋白，生物活性验证显示可诱导软骨细胞分化。
3. **文献名称**：Recombinant IHH Enhances Tumor Angiogenesis in Colorectal Cancer Models
**作者**：L. Chen et al.
**摘要**：利用大肠癌小鼠模型揭示重组IHH蛋白通过上调VEGF表达促进肿瘤血管生成，提示靶向IHH可能成为癌症治疗新策略。
*注：以上文献信息为示例性质，实际引用需核实具体论文来源。建议通过PubMed或Web of Science以"recombinant IHH protein"为关键词检索最新研究。*
Indian Hedgehog (IHH) is a secreted signaling protein belonging to the Hedgehog (Hh) family, which plays critical roles in embryonic development, tissue homeostasis, and regeneration. Discovered as a homolog of the Drosophila Hedgehog protein, IHH is one of three mammalian Hh proteins, alongside Sonic Hedgehog (SHH) and Desert Hedgehog (DHH). It is particularly essential in endochondral ossification, regulating chondrocyte proliferation, differentiation, and bone growth. IHH coordinates with parathyroid hormone-related protein (PTHrP) to maintain the balance between proliferating and hypertrophic chondrocytes in growth plates, ensuring proper skeletal development. Dysregulation of IHH signaling is linked to skeletal disorders, such as brachydactyly type A1. and cancers, including chondrosarcoma.
Recombinant IHH protein is engineered using biotechnological platforms (e.g., bacterial, insect, or mammalian expression systems) to produce bioactive forms for research and therapeutic applications. This involves cloning the IHH gene into expression vectors, followed by purification via affinity chromatography (e.g., His-tag). The recombinant protein retains the ability to bind Patched (PTCH1), the primary Hh receptor, initiating downstream signaling through Smoothened (SMO) and GLI transcription factors. Researchers utilize recombinant IHH to study Hh pathway mechanisms, screen modulators (e.g., SMO inhibitors like cyclopamine derivatives), and explore regenerative therapies for bone/cartilage defects. Its applications extend to disease modeling, drug discovery, and potential clinical use in tissue engineering, underscoring its versatility in both basic and translational studies.
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