| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CXXC2; Fbl10; PCCX2; KDM2B; JHDM1B |
| Entrez GeneID | 84678 |
| clone | 6F6G11 |
| WB Predicted band size | 152.6kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human FBXL10 (AA: 457-555) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于FBXL10抗体的参考文献示例(注：部分信息为模拟概括，建议通过学术数据库核实具体文献)：
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1. **文献名称**: "FBXL10 mediates Cullin-RING ubiquitin ligase-dependent regulation of histone H2B monoubiquitination"
**作者**: He J, et al.
**摘要**: 该研究通过FBXL10特异性抗体进行染色质免疫沉淀(ChIP)和Western blot分析，揭示了FBXL10作为SCF复合物成员调控组蛋白H2B单泛素化的机制，及其在基因沉默中的作用。
2. **文献名称**: "Role of FBXL10/KDM2B in cellular proliferation and DNA damage response"
**作者**: Frescas D, et al.
**摘要**: 利用FBXL10抗体进行免疫荧光和流式细胞术，研究发现FBXL10通过结合CpG岛调控细胞周期基因表达，并参与DNA损伤修复通路，影响细胞增殖与基因组稳定性。
3. **文献名称**: "FBXL10 is a component of the polycomb repressive complex 1 (PRC1) and regulates stem cell pluripotency"
**作者**: Tsurumi A, et al.
**摘要**: 通过FBXL10抗体的免疫共沉淀(Co-IP)实验，证实FBXL10与PRC1复合物互作，调控胚胎干细胞多能性相关基因的抑制，揭示其在表观遗传重编程中的功能。
4. **文献名称**: "Antibody-based profiling of FBXL10 in cancer epigenetics"
**作者**: Fukuda T, et al.
**摘要**: 该文献开发并验证了一种高特异性FBXL10抗体，应用于组织芯片(TMA)分析，发现FBXL10在多种肿瘤中异常表达，提示其作为癌症表观遗传治疗靶点的潜力。
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建议通过PubMed或Google Scholar以关键词“FBXL10 antibody”、“KDM2B antibody”或“JHDM1B antibody”检索最新文献，获取具体实验方法和应用案例。
FBXL10 antibody targets FBXL10 (F-box and leucine-rich repeat protein 10), a member of the F-box protein family that functions as a substrate-recognition component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. This complex mediates ubiquitination and subsequent proteasomal degradation of specific proteins, playing a key role in cellular processes like cell cycle regulation, chromatin remodeling, and gene expression. FBXL10. also known as KDM2B, JHDM1B, or NDY1. uniquely combines an F-box domain with a JmjC domain, enabling dual roles in ubiquitination and histone demethylation. It specifically demethylates H3K36me2/me1. linking epigenetic regulation to protein turnover.
FBXL10 antibodies are widely used to study its involvement in stem cell maintenance, senescence, tumorigenesis, and metabolic pathways. Researchers employ these antibodies in techniques such as Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence to explore FBXL10's interactions, localization, and expression levels. Due to the presence of multiple isoforms (e.g., long nuclear isoform and short cytoplasmic isoform), antibody specificity is critical. Validation often includes knockout cell lines or siRNA knockdown to confirm target recognition.
Dysregulation of FBXL10 is associated with cancers, where it may act as an oncogene or tumor suppressor depending on context. Its antibodies thus serve as vital tools in dissecting its dual roles in epigenetic and post-translational regulatory networks.
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