Mouse Monoclonal ULK2 Antibody

The ULK2 (Unc-51-like kinase 2) antibody is a crucial tool for studying the ULK2 protein, a serine/threonine kinase central to autophagy regulation. ULK2. along with its homolog ULK1. forms the core of the autophagy initiation complex, which responds to cellular stress, nutrient deprivation, or mTOR (mechanistic target of rapamycin) pathway inhibition. While ULK1 is predominantly studied, ULK2 shares structural homology and partially redundant functions, though its expression varies across tissues. The antibody enables detection of ULK2's expression, post-translational modifications (e.g., phosphorylation at specific residues like Ser757), and interactions with partners such as ATG13. FIP200. or AMPK. Researchers use ULK2-specific antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to dissect its role in autophagosome formation, lysosomal degradation, and crosstalk with pathways like PI3K-AKT-mTOR. Dysregulation of ULK2 is implicated in cancer (acting as tumor suppressor or promoter depending on context), neurodegenerative diseases, and metabolic disorders. However, antibody specificity remains critical due to ULK1/ULK2 sequence similarity. Validation via knockout controls or peptide competition ensures accurate interpretation, particularly when studying tissue-specific autophagy mechanisms or therapeutic targeting of the ULK kinase family.