| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ATG1B; Unc51.2 |
| Entrez GeneID | 9706 |
| clone | 2H4B2 |
| WB Predicted band size | 112.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant fragment of human ULK2 (AA: 1-155) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3条关于ULK2抗体的参考文献示例(注：文献为虚拟示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*"Development and validation of a ULK2-specific monoclonal antibody for autophagy research"*
**作者**：Zhang Y, et al.
**摘要**：本研究报道了一种新型ULK2单克隆抗体的开发与验证，通过Western blot和免疫荧光证实其特异性，可用于检测哺乳动物细胞中内源性ULK2蛋白的表达及亚细胞定位。
2. **文献名称**：*"ULK2 modulates autophagosome formation through phosphorylation of ATG13"*
**作者**：Tanaka S, Mizushima N.
**摘要**：利用ULK2特异性抗体研究其与ATG13的相互作用，发现ULK2在营养缺失条件下通过磷酸化ATG13调控自噬体形成，提示其与ULK1的功能互补性。
3. **文献名称**：*"Differential expression of ULK2 in neurodegenerative disorders revealed by immunohistochemistry"*
**作者**：Wang L, et al.
**摘要**：通过ULK2抗体对阿尔茨海默病和帕金森病患者脑组织进行免疫组化分析，发现ULK2蛋白表达水平与病理蛋白聚集呈负相关，提示其潜在神经保护作用。
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**建议**：如需真实文献，可在PubMed或Google Scholar中搜索关键词“ULK2 antibody”、“ULK2 autophagy”或“ULK2 kinase”，并筛选近5年高引用研究。
The ULK2 antibody is a research tool designed to detect Unc-51-like kinase 2 (ULK2), a serine/threonine kinase critical in autophagy regulation. ULK2. homologous to ULK1. forms complexes with autophagy-related proteins (ATG13. FIP200. ATG101) to initiate autophagosome formation. It integrates stress signals via upstream regulators like mTORC1 and AMPK, which phosphorylate ULK2 to modulate its activity. While ULK1 is dominant in autophagy, ULK2 exhibits overlapping functions and tissue-specific roles, particularly in contexts where ULK1 is absent or dysregulated.
ULK2 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and post-translational modifications (e.g., phosphorylation at Ser757 or Ser758). These antibodies often target specific epitopes, such as the N-terminal kinase domain or C-terminal region. Validation includes knockout/knockdown controls to confirm specificity.
Research applications span cancer, neurodegeneration, and metabolic disorders, as ULK2 dysregulation links to autophagy-related pathologies. Commercial ULK2 antibodies vary in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and reactivity (human, mouse, rat). Researchers must select antibodies based on experimental needs, emphasizing validation data to ensure reliability. Understanding ULK2's role enhances insights into autophagy mechanisms and therapeutic targeting in disease.
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